8W8Q

Cryo-EM structure of the GPR101-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Soluble cytochrome b562,Probable G-protein coupled receptor 101	polymer	655	73588.9	1	UniProt (P0ABE7) UniProt (Q96P66) Pfam (PF00001)	Escherichia coli	Cytochrome b-562
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (N)	Nb35	polymer	128	13885.4	1		Homo sapiens (human)

Sequence modifications

C: -105 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -146	-	initiating methionine
Lys -145	-	expression tag
Thr -144	-	expression tag
Ile -143	-	expression tag
Ile -142	-	expression tag
Ala -141	-	expression tag
Leu -140	-	expression tag
Ser -139	-	expression tag
Tyr -138	-	expression tag
Ile -137	-	expression tag
Phe -136	-	expression tag
Cys -135	-	expression tag
Leu -134	-	expression tag
Val -133	-	expression tag
Phe -132	-	expression tag
Ala -131	-	expression tag
Asp -130	-	expression tag
Tyr -129	-	expression tag
Lys -128	-	expression tag
Asp -127	-	expression tag
Asp -126	-	expression tag
Asp -125	-	expression tag
Asp -124	-	expression tag
Lys -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
Glu -112	-	expression tag
Asn -111	-	expression tag
Leu -110	-	expression tag
Tyr -109	-	expression tag
Phe -108	-	expression tag
Gln -107	-	expression tag
Gly -106	-	expression tag
Trp -99	Met 29	conflict
Ile -4	His 124	conflict
Leu 0	Arg 128	conflict

C: 1 - 508 (UniProt: Q96P66)
A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Phe 60	Met 60	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	176834.8
All*	Total formula weight	176834.8

*Water molecules are not included.