8W8H

2-Ketoglutarate-Dependent Dioxygenase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	2OG-Fe dioxygenase family protein	polymer	263	29672.1	4	UniProt (A0A3A5I123) In PDB	Bacillus licheniformis
2	A, B, C, D	ISOLEUCINE	non-polymer		131.2	8	Chemie (ILE)

Sequence modifications

A, B, C, D: 2 - 229 (UniProt: A0A3A5I123)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag
Ala 18	Ser 32	engineered mutation
Lys 164	Gln 178	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	118688.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1049.4
All*	Total formula weight	119737.7

*Water molecules are not included.