8W8H
2-Ketoglutarate-Dependent Dioxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-13 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.145, 129.628, 153.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.650 - 3.200 |
| R-factor | 0.21863 |
| Rwork | 0.216 |
| R-free | 0.26674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.650 | 3.460 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.227 | 0.606 |
| Number of reflections | 15586 | 3153 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 17 mg/mL enzyme was incubated with 9.2 mM L-Ile and grew at 1 M sodium citrate for 15 days |
