8W88

Cryo-EM structure of the SEP363856-bound TAAR1-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45755.5	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody35	polymer	139	15271.9	1		Lama glama
5	R	Trace amine-associated receptor 1	polymer	339	39130.6	1	UniProt (Q96RJ0) Pfam (PF00001) In PDB	Homo sapiens (human)	TaR-1,Trace amine receptor 1
6	R	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)
7	R	1-[(7~{S})-5,7-dihydro-4~{H}-thieno[2,3-c]pyran-7-yl]-~{N}-methyl-methanamine	non-polymer		183.3	1	Chemie (UJL)

Sequence modifications

A: 0 - 393 (UniProt: P63092)

PDB	External Database	Details
Ala 225	Gly 226	conflict
Ser 365	Ala 366	variant

B: 15 - 353 (UniProt: P62873)

PDB	External Database	Details
Met 9	-	initiating methionine
Gly 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Gln 14	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145934.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1343.2
All*	Total formula weight	147278.0

*Water molecules are not included.