8W51
Structure of YchF(H114A) on E.coli 50S ribosomal subunit
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 23S rRNA | polymer | 2904 | 941761.6 | 1 | Escherichia coli | ||
| 2 | B (B) | 5S rRNA | polymer | 118 | 38177.8 | 1 | GenBank (1727529157) | Escherichia coli | |
| 3 | C (C) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 4 | D (D) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (C3SQU2) Pfam (PF00297) UniProt (by SIFTS) (P60438) | Escherichia coli | |
| 5 | E (E) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli | Large ribosomal subunit protein uL4 |
| 6 | F (F) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli | Large ribosomal subunit protein uL5 |
| 7 | G (G) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli | Large ribosomal subunit protein uL6 |
| 8 | H (H) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) | Escherichia coli | Large ribosomal subunit protein bL9 |
| 9 | I (J) | Large ribosomal subunit protein uL11 | polymer | 142 | 14894.4 | 1 | UniProt (A7ZUJ6) Pfam (PF03946) Pfam (PF00298) UniProt (by SIFTS) (P0A7J7) | Escherichia coli | 50S ribosomal protein L11 |
| 10 | J (K) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli | Large ribosomal subunit protein uL13 |
| 11 | K (L) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli | Large ribosomal subunit protein uL14 |
| 12 | L (M) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli | Large ribosomal subunit protein uL15 |
| 13 | M (N) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 14 | N (O) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli | Large ribosomal subunit protein bL17 |
| 15 | O (P) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli | Large ribosomal subunit protein uL18 |
| 16 | P (Q) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli | Large ribosomal subunit protein bL19 |
| 17 | Q (R) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli | Large ribosomal subunit protein bL20 |
| 18 | R (S) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli | Large ribosomal subunit protein bL21 |
| 19 | S (T) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli | Large ribosomal subunit protein uL22 |
| 20 | T (U) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli | Large ribosomal subunit protein uL23 |
| 21 | U (V) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli | Large ribosomal subunit protein uL24 |
| 22 | V (W) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli | Large ribosomal subunit protein bL25 |
| 23 | W (X) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli | Large ribosomal subunit protein bL27 |
| 24 | X (Y) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli | Large ribosomal subunit protein bL28 |
| 25 | Y (Z) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli | Large ribosomal subunit protein uL29 |
| 26 | Z (0) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli | Large ribosomal subunit protein uL30 |
| 27 | AA (1) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli | Large ribosomal subunit protein bL32 |
| 28 | BA (2) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli | Large ribosomal subunit protein bL33 |
| 29 | CA (3) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli | Large ribosomal subunit protein bL34 |
| 30 | DA (4) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 31 | EA (5) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 32 | FA (y) | Ribosome-binding ATPase YchF | polymer | 379 | 41651.3 | 1 | UniProt (P0ABU2) Pfam (PF01926) Pfam (PF06071) | Escherichia coli | |
| 33 | AB, AC, AD, AE, BB... (A, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 104 | Chemie (MG) | |||
| 34 | GE (5) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
y: 1 - 363 (UniProt: P0ABU2)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 114 | His 114 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 32 |
| Total formula weight | 1398745.2 | |
| Non-Polymers* | Number of molecules | 105 |
| Total formula weight | 2593.1 | |
| All* | Total formula weight | 1401338.3 |
