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- EMDB-37271: Structure of YchF(H114A) on E.coli 50S ribosomal subunit -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-37271

Title

Structure of YchF(H114A) on E.coli 50S ribosomal subunit

Map data

Sample

Complex: Complex of 50S subunit and YchF
- Complex: 50S subunit
  - RNA: x 2 types
  - Protein or peptide: x 29 types
- Complex: YchF
  - Protein or peptide: x 1 types
Ligand: x 2 types

Keywords

YchF / OLA1 / Ribosome

Function / homology

Function and homology information

ribosomal large subunit binding / enzyme inhibitor activity / transcriptional attenuation / positive regulation of ribosome biogenesis / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of DNA-templated DNA replication initiation ...
Similarity search - Function

Ribosome-binding ATPase YchF/Obg-like ATPase 1 / YchF, C-terminal domain / TGS-like domain superfamily / YchF, N-terminal / Protein of unknown function (DUF933) / TGS-like / TGS domain profile. / TGS / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. ...Ribosome-binding ATPase YchF/Obg-like ATPase 1 / YchF, C-terminal domain / TGS-like domain superfamily / YchF, N-terminal / Protein of unknown function (DUF933) / TGS-like / TGS domain profile. / TGS / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / 50S ribosome-binding GTPase / GTP binding domain / Beta-grasp domain superfamily / Ribosomal protein L11, bacterial-type / Ribosomal protein L25, short-form / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / : / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / : / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L17 signature. / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L30, bacterial-type / : / L28p-like / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 superfamily / Ribosomal protein L21
Similarity search - Domain/homology

Biological species

Escherichia coli (E. coli)

Method

single particle reconstruction / cryo EM / Resolution: 2.4 Å

Authors

Yu T / Li X / Zeng F

Funding support

China, 3 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)	32171200	China
Guangdong Basic and Applied Basic Research Foundation	2021A1515010805	China
Shenzhen Science and Technology Program	JCYJ20220530115210023	China

Citation

Journal: To Be Published
Title: Structure basis of translation regulation by YchF bound to ribosome
Authors: Li X / Yu T / Li Q / Zeng F

History

Deposition	Aug 25, 2023	-
Header (metadata) release	Aug 28, 2024	-
Map release	Aug 28, 2024	-
Update	Aug 28, 2024	-
Current status	Aug 28, 2024	Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images	emd_37271.png

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Downloads & links

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EMDB archive

Map data	emd_37271.map.gz	175 MB		EMDB map data format
Header (meta data)	emd-37271-v30.xml emd-37271.xml	51.4 KB 51.4 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_37271_fsc.xml	13.5 KB	Display	FSC data file
Images	emd_37271.png	47.8 KB
Filedesc metadata	emd-37271.cif.gz	10.6 KB
Others	emd_37271_half_map_1.map.gz emd_37271_half_map_2.map.gz	187.8 MB 187.4 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-37271 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37271	HTTPS FTP

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Validation report

Summary document	emd_37271_validation.pdf.gz	1000.1 KB	Display	EMDB validaton report
Full document	emd_37271_full_validation.pdf.gz	999.6 KB	Display
Data in XML	emd_37271_validation.xml.gz	21 KB	Display
Data in CIF	emd_37271_validation.cif.gz	27.6 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37271 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37271	HTTPS FTP

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Related structure data

Related structure data	8w51MC 37258C 37263C 8kieC C: citing same article (ref.) M: atomic model generated by this map
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (45) #157 - Jan 2013 Transfer-Messenger RNA similarity (45) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (43) #121 - Jan 2010 70S Ribosomes similarity (10) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (4) #117 - Sep 2009 Xanthine Oxidoreductase similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (2) #154 - Oct 2012 Citric Acid Cycle similarity (1) #82 - Oct 2006 Cytochrome p450 similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

EMDB pages

EMDB (EBI/PDBe) /

EMDataResource

Related items in Molecule of the Month

#253 - Jan 2021
Expressome
similarity (45)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (45)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (43)
#121 - Jan 2010
70S Ribosomes
similarity (10)
#16 - Apr 2001
Aminoacyl-tRNA Synthetases
similarity (4)
#117 - Sep 2009
Xanthine Oxidoreductase
similarity (1)
#266 - Feb 2022
Oligosaccharyltransferase
similarity (2)
#154 - Oct 2012
Citric Acid Cycle
similarity (1)
#82 - Oct 2006
Cytochrome p450
similarity (1)
#241 - Jan 2020
Twenty Years of Molecules
similarity (1)

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Map

File

Download / File: emd_37271.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Projections & slices

Image control

Size
Brightness
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Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	1.05 Å/pix. x 384 pix. = 403.2 Å	1.05 Å/pix. x 384 pix. = 403.2 Å	1.05 Å/pix. x 384 pix. = 403.2 Å
Surface
Projections
Slices (1/3)
Slices (1/2)
Slices (2/3)

Images are generated by Spider.

Voxel size

X=Y=Z: 1.05 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.006
Minimum - Maximum	-0.042521127 - 0.10259886
Average (Standard dev.)	0.000012258462 (±0.003115112)

Symmetry

Space group: 1

Details

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Supplemental data

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Half map: #2

File

emd_37271_half_map_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

File

emd_37271_half_map_2.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of 50S subunit and YchF

Entire	Name: Complex of 50S subunit and YchF
Components	Complex: Complex of 50S subunit and YchF Complex: 50S subunit RNA: 23S rRNA RNA: 5S rRNA Protein or peptide: 50S ribosomal protein L2 Protein or peptide: 50S ribosomal protein L3 Protein or peptide: 50S ribosomal protein L4 Protein or peptide: 50S ribosomal protein L5 Protein or peptide: 50S ribosomal protein L6 Protein or peptide: 50S ribosomal protein L9 Protein or peptide: Large ribosomal subunit protein uL11 Protein or peptide: 50S ribosomal protein L13 Protein or peptide: 50S ribosomal protein L14 Protein or peptide: 50S ribosomal protein L15 Protein or peptide: 50S ribosomal protein L16 Protein or peptide: 50S ribosomal protein L17 Protein or peptide: 50S ribosomal protein L18 Protein or peptide: 50S ribosomal protein L19 Protein or peptide: 50S ribosomal protein L20 Protein or peptide: 50S ribosomal protein L21 Protein or peptide: 50S ribosomal protein L22 Protein or peptide: 50S ribosomal protein L23 Protein or peptide: 50S ribosomal protein L24 Protein or peptide: 50S ribosomal protein L25 Protein or peptide: 50S ribosomal protein L27 Protein or peptide: 50S ribosomal protein L28 Protein or peptide: 50S ribosomal protein L29 Protein or peptide: 50S ribosomal protein L30 Protein or peptide: 50S ribosomal protein L32 Protein or peptide: 50S ribosomal protein L33 Protein or peptide: 50S ribosomal protein L34 Protein or peptide: 50S ribosomal protein L35 Protein or peptide: 50S ribosomal protein L36 Complex: YchF Protein or peptide: Ribosome-binding ATPase YchF Ligand: MAGNESIUM ION Ligand: ZINC ION

Entire

Name: Complex of 50S subunit and YchF

Components

Complex: Complex of 50S subunit and YchF
- Complex: 50S subunit
  - RNA: 23S rRNA
  - RNA: 5S rRNA
  - Protein or peptide: 50S ribosomal protein L2
  - Protein or peptide: 50S ribosomal protein L3
  - Protein or peptide: 50S ribosomal protein L4
  - Protein or peptide: 50S ribosomal protein L5
  - Protein or peptide: 50S ribosomal protein L6
  - Protein or peptide: 50S ribosomal protein L9
  - Protein or peptide: Large ribosomal subunit protein uL11
  - Protein or peptide: 50S ribosomal protein L13
  - Protein or peptide: 50S ribosomal protein L14
  - Protein or peptide: 50S ribosomal protein L15
  - Protein or peptide: 50S ribosomal protein L16
  - Protein or peptide: 50S ribosomal protein L17
  - Protein or peptide: 50S ribosomal protein L18
  - Protein or peptide: 50S ribosomal protein L19
  - Protein or peptide: 50S ribosomal protein L20
  - Protein or peptide: 50S ribosomal protein L21
  - Protein or peptide: 50S ribosomal protein L22
  - Protein or peptide: 50S ribosomal protein L23
  - Protein or peptide: 50S ribosomal protein L24
  - Protein or peptide: 50S ribosomal protein L25
  - Protein or peptide: 50S ribosomal protein L27
  - Protein or peptide: 50S ribosomal protein L28
  - Protein or peptide: 50S ribosomal protein L29
  - Protein or peptide: 50S ribosomal protein L30
  - Protein or peptide: 50S ribosomal protein L32
  - Protein or peptide: 50S ribosomal protein L33
  - Protein or peptide: 50S ribosomal protein L34
  - Protein or peptide: 50S ribosomal protein L35
  - Protein or peptide: 50S ribosomal protein L36
- Complex: YchF
  - Protein or peptide: Ribosome-binding ATPase YchF
Ligand: MAGNESIUM ION
Ligand: ZINC ION

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Supramolecule #1: Complex of 50S subunit and YchF

Supramolecule	Name: Complex of 50S subunit and YchF / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#32

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Supramolecule #2: 50S subunit

Supramolecule	Name: 50S subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#31
Source (natural)	Organism: Escherichia coli (E. coli)

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Supramolecule #3: YchF

Supramolecule	Name: YchF / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #32
Source (natural)	Organism: Escherichia coli (E. coli)

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Macromolecule #1: 23S rRNA

Macromolecule	Name: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 941.761625 KDa
Sequence	String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG ...String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAU(1MG)(PSU)(5MU)GAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCA AU CAAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACU G UUUCGGCAAG GGGGUCAACC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACAC GGCGGG(PSU)GCU AACGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUG GGA AACGAUGUGG GAAGGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCAC UG GUCGAGUCGG CCUGCGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCUUAU GCGUUGUU G GGUAGGGGAG CGUUCUGUAA GCCUGCGAAG GUGUGCUGUG AGGCAUGCUG GAGGUAUCAG AAGUGCGAAU GCUGACAUA AGUAACGAUA AAGCGGGUGA AAAGCCCGCU CGCCGGAAGA CCAAGGGUUC CUGUCCAACG UUAAUCGGGG CAGGGUGAGU CGACCCCUA AGGCGAGGCC GAAAGGCGUA GUCGAUGGGA AACAGGUUAA UAUUCCUGUA CUUGGUGUUA CUGCGAAGGG G GGACGGAG AAGGCUAUGU UGGCCGGGCG ACGGUUGUCC CGGUUUAAGC GUGUAGGCUG GUUUUCCAGG CAAAUCCGGA AA AUCAAGG CUGAGGCGUG AUGACGAGGC ACUACGGUGC UGAAGCAACA AAUGCCCUGC UUCCAGGAAA AGCCUCUAAG CAU CAGGUA ACAUCAAAUC GUACCCCAAA CCGACACAGG UGGUCAGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGA AGGAA CUAGGCAAAA UGGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA GCUGA AAUC AGUCGAAGAU ACCAGCUGGC UGCAACUGUU UAUUAAAAAC ACAGCACUGU GCAAACACGA AAGUGGACGU AUACGG UGU GACGCCU(2MG)CC CGGUGCCGGA AGGUUAAUUG AUGGGGUUAG CGCAAGCGAA GCUCUUGAUC GAAGCCCCGG UAA ACGGCG GCCG(PSU)AACUA (PSU)AACGGUCCU AAGGUAGCGA AA(5MU)UCCUUGU CGGGUAAGUU CCGAC(5MC)UG C ACGAAUGGCG UAAUGAUGGC CAGGCUGUCU CCACCCGAGA CUCAGUGAAA UUGAACUCGC UGUGAAGAUG CAGUGUACC CGCGGCAAGA CGGAAAGACC CCGUGAACCU UUACUAUAGC UUGACACUGA ACAUUGAGCC UUGAUGUGUA GGAUAGGUGG GAGGCUUUG AAGUGUGGAC GCCAGUCUGC AUGGAGCCGA CCUUGAAAUA CCACCCUUUA AUGUUUGAUG UUCUAACGUU G ACCCGUAA UCCGGGUUGC GGACAGUGUC UGGUGGGUAG UUUGACUG(OMG)G GCGGUCUCCU CCUAAAGAGU AACGGAGG A GCACGAAGGU UGGCUAAUCC UGGUCGGACA UCAGGAGGUU AGUGCAAUGG CAUAAGCCAG CUUGACUGCG AGCGUGACG GCGCGAGCAG GUGCGAAAGC AGGUCAUAGU GAUCCGGUGG UUCUGAAUGG AAGGGCCAUC GCUCAACGGA UAAAAGGUAC UCCG(2MG)GGAU AACAGGC(PSU)GA UACCGCCCAA GAGUUCAUAU CGACGGCGGU GUUUGGCA(OMC)C UCGA(PSU) GUCG GCUCAUCACA UCCUGGGGCU GAAGUAGGUC CCAAGGGUAU GGC(OMU)GUUCGC CAUUUAAAGU GGUACGCGAG C (PSU)GGGUUUA GAACGUCGUG AGACAGU(PSU)CG GUCCCUAUCU GCCGUGGGCG CUGGAGAACU GAGGGGGGCU GCUC CUAGU ACGAGAGGAC CGGAGUGGAC GCAUCACUGG UGUUCGGGUU GUCAUGCCAA UGGCACUGCC CGGUAGCUAA AUGCG GAAG AGAUAAGUGC UGAAAGCAUC UAAGCACGAA ACUUGCCCCG AGAUGAGUUC UCCCUGACCC UUUAAGGGUC CUGAAG GAA CGUUGAAGAC GACGACGUUG AUAGGCCGGG UGUGUAAGCG CAGCGAUGCG UUGAGCUAAC CGGUACUAAU GAACCGU GA GGCUUAACCU U

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Macromolecule #2: 5S rRNA

Macromolecule	Name: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 38.177762 KDa
Sequence	String: GCCUGGCGGC CGUAGCGCGG UGGUCCCACC UGACCCCAUG CCGAACUCAG AAGUGAAACG CCGUAGCGCC GAUGGUAGUG UGGGGUCUC CCCAUGCGAG AGUAGGGAAC UGCCAGGCA GENBANK: GENBANK: CP019213.3

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Macromolecule #3: 50S ribosomal protein L2

Macromolecule	Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 29.923619 KDa
Sequence	String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD ...String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: 50S ribosomal protein L3

Macromolecule	Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 22.277535 KDa
Sequence	String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE ...String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: 50S ribosomal protein L4

Macromolecule	Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 22.121566 KDa
Sequence	String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD ...String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: 50S ribosomal protein L5

Macromolecule	Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 20.333611 KDa
Sequence	String: MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: 50S ribosomal protein L6

Macromolecule	Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 18.932791 KDa
Sequence	String: MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: 50S ribosomal protein L9

Macromolecule	Name: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.78902 KDa
Sequence	String: MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #9: Large ribosomal subunit protein uL11

Macromolecule	Name: Large ribosomal subunit protein uL11 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 14.894362 KDa
Sequence	String: MAKKVQAYVK LQVAAGMANP SPPVGPALGQ QGVNIMEFCK AFNAKTDSIE KGLPIPVVIT VYADRSFTFV TKTPPAAVLL KKAAGIKSG SGKPNKDKVG KISRAQLQEI AQTKAADMTG ADIEAMTRSI EGTARSMGLV VED UniProtKB: Large ribosomal subunit protein uL11

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Macromolecule #10: 50S ribosomal protein L13

Macromolecule	Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 16.050606 KDa
Sequence	String: MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #11: 50S ribosomal protein L14

Macromolecule	Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.565067 KDa
Sequence	String: MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL UniProtKB: Large ribosomal subunit protein uL14

+
Macromolecule #12: 50S ribosomal protein L15

Macromolecule	Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.008471 KDa
Sequence	String: MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #13: 50S ribosomal protein L16

Macromolecule	Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.312269 KDa
Sequence	String: MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV RMGKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM UniProtKB: Large ribosomal subunit protein uL16

+
Macromolecule #14: 50S ribosomal protein L17

Macromolecule	Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 14.393657 KDa
Sequence	String: MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE UniProtKB: Large ribosomal subunit protein bL17

+
Macromolecule #15: 50S ribosomal protein L18

Macromolecule	Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 12.794668 KDa
Sequence	String: MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #16: 50S ribosomal protein L19

Macromolecule	Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.159278 KDa
Sequence	String: MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN UniProtKB: Large ribosomal subunit protein bL19

+
Macromolecule #17: 50S ribosomal protein L20

Macromolecule	Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.528024 KDa
Sequence	String: MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA UniProtKB: Large ribosomal subunit protein bL20

+
Macromolecule #18: 50S ribosomal protein L21

Macromolecule	Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.586374 KDa
Sequence	String: MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #19: 50S ribosomal protein L22

Macromolecule	Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 12.253359 KDa
Sequence	String: METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #20: 50S ribosomal protein L23

Macromolecule	Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.22216 KDa
Sequence	String: MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #21: 50S ribosomal protein L24

Macromolecule	Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.33925 KDa
Sequence	String: MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #22: 50S ribosomal protein L25

Macromolecule	Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 10.713465 KDa
Sequence	String: MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA UniProtKB: Large ribosomal subunit protein bL25

+
Macromolecule #23: 50S ribosomal protein L27

Macromolecule	Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.14654 KDa
Sequence	String: MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE UniProtKB: Large ribosomal subunit protein bL27

+
Macromolecule #24: 50S ribosomal protein L28

Macromolecule	Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.027551 KDa
Sequence	String: MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY UniProtKB: Large ribosomal subunit protein bL28

+
Macromolecule #25: 50S ribosomal protein L29

Macromolecule	Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.286464 KDa
Sequence	String: MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #26: 50S ribosomal protein L30

Macromolecule	Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.55482 KDa
Sequence	String: MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #27: 50S ribosomal protein L32

Macromolecule	Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.463445 KDa
Sequence	String: MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK UniProtKB: Large ribosomal subunit protein bL32

+
Macromolecule #28: 50S ribosomal protein L33

Macromolecule	Name: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.388631 KDa
Sequence	String: MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK UniProtKB: Large ribosomal subunit protein bL33

+
Macromolecule #29: 50S ribosomal protein L34

Macromolecule	Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 5.397463 KDa
Sequence	String: MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK UniProtKB: Large ribosomal subunit protein bL34

+
Macromolecule #30: 50S ribosomal protein L35

Macromolecule	Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.313032 KDa
Sequence	String: MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA UniProtKB: Large ribosomal subunit protein bL35

+
Macromolecule #31: 50S ribosomal protein L36

Macromolecule	Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 4.37739 KDa
Sequence	String: MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG UniProtKB: Large ribosomal subunit protein bL36A

+
Macromolecule #32: Ribosome-binding ATPase YchF

Macromolecule	Name: Ribosome-binding ATPase YchF / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 41.651305 KDa
Recombinant expression	Organism: Escherichia coli (E. coli)
Sequence	String: MGHHHHHHEN LYFQGHMGFK CGIVGLPNVG KSTLFNALTK AGIEAANFPF CTIEPNTGVV PMPDPRLDQL AEIVKPQRTL PTTMEFVDI AGLVKGASKG EGLGNQFLTN IRETEAIGHV VRCFENDNII AVSGKVNPAD DIEVINTELA LADLDTCERA I HRVQKKAK ...String: MGHHHHHHEN LYFQGHMGFK CGIVGLPNVG KSTLFNALTK AGIEAANFPF CTIEPNTGVV PMPDPRLDQL AEIVKPQRTL PTTMEFVDI AGLVKGASKG EGLGNQFLTN IRETEAIGHV VRCFENDNII AVSGKVNPAD DIEVINTELA LADLDTCERA I HRVQKKAK GGDKDAKAEL AVLEKCLPQL ENAGMLRALD LSAEEKAAIR YLSFLTLKPT MYIANVNEDG FENNPYLDQV RE IAAKEGS VVVPVCAAVE ADIAELDDEE RDEFMQELGL EEPGLNRVIR AGYKLLNLQT YFTAGVKEVR AWTIPVGATA PQA AGKIHT DFEKGFIRAQ TISFEDFITY KGEQGAKEAG KMRAEGKDYI VKDGDVMNFL FNV UniProtKB: Ribosome-binding ATPase YchF

+
Macromolecule #33: MAGNESIUM ION

+
Macromolecule #34: ZINC ION

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer	pH: 7.5
Vitrification	Cryogen name: ETHANE

-
Electron microscopy

Microscope	FEI TITAN KRIOS
Image recording	Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 30.0 e/Å²
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup model	Type of model: PDB ENTRY PDB model - PDB ID: 5u4i
Final reconstruction	Resolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 137631
Initial angle assignment	Type: ANGULAR RECONSTITUTION
Final angle assignment	Type: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

+
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Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 33 / Number of copies: 104 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

Macromolecule	Name: ZINC ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: ZN
Molecular weight	Theoretical: 65.409 Da

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-Supplemental data

-Half map: #2

-Half map: #1

-Sample components

+Entire : Complex of 50S subunit and YchF

+Supramolecule #1: Complex of 50S subunit and YchF

+Supramolecule #2: 50S subunit

+Supramolecule #3: YchF

+Macromolecule #1: 23S rRNA

+Macromolecule #2: 5S rRNA

+Macromolecule #3: 50S ribosomal protein L2

+Macromolecule #4: 50S ribosomal protein L3

+Macromolecule #5: 50S ribosomal protein L4

+Macromolecule #6: 50S ribosomal protein L5

+Macromolecule #7: 50S ribosomal protein L6

+Macromolecule #8: 50S ribosomal protein L9

+Macromolecule #9: Large ribosomal subunit protein uL11

+Macromolecule #10: 50S ribosomal protein L13

+Macromolecule #11: 50S ribosomal protein L14

+Macromolecule #12: 50S ribosomal protein L15

+Macromolecule #13: 50S ribosomal protein L16

+Macromolecule #14: 50S ribosomal protein L17

+Macromolecule #15: 50S ribosomal protein L18

+Macromolecule #16: 50S ribosomal protein L19

+Macromolecule #17: 50S ribosomal protein L20

+Macromolecule #18: 50S ribosomal protein L21

+Macromolecule #19: 50S ribosomal protein L22

+Macromolecule #20: 50S ribosomal protein L23

+Macromolecule #21: 50S ribosomal protein L24

+Macromolecule #22: 50S ribosomal protein L25

+Macromolecule #23: 50S ribosomal protein L27

+Macromolecule #24: 50S ribosomal protein L28

+Macromolecule #25: 50S ribosomal protein L29

+Macromolecule #26: 50S ribosomal protein L30

+Macromolecule #27: 50S ribosomal protein L32

+Macromolecule #28: 50S ribosomal protein L33

+Macromolecule #29: 50S ribosomal protein L34

+Macromolecule #30: 50S ribosomal protein L35

+Macromolecule #31: 50S ribosomal protein L36

+Macromolecule #32: Ribosome-binding ATPase YchF

+Macromolecule #33: MAGNESIUM ION

+Macromolecule #34: ZINC ION

-Experimental details

-Structure determination

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Movie

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Viewer log

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This page

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This web site

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Options

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Open data

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Basic information

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Downloads & links

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Related structure data

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Links

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Map

Image control

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Supplemental data

-
Half map: #2

-
Half map: #1

-
Sample components

+
Entire : Complex of 50S subunit and YchF

+
Supramolecule #1: Complex of 50S subunit and YchF

+
Supramolecule #2: 50S subunit

+
Supramolecule #3: YchF

+
Macromolecule #1: 23S rRNA

+
Macromolecule #2: 5S rRNA

+
Macromolecule #3: 50S ribosomal protein L2

+
Macromolecule #4: 50S ribosomal protein L3

+
Macromolecule #5: 50S ribosomal protein L4

+
Macromolecule #6: 50S ribosomal protein L5

+
Macromolecule #7: 50S ribosomal protein L6

+
Macromolecule #8: 50S ribosomal protein L9

+
Macromolecule #9: Large ribosomal subunit protein uL11

+
Macromolecule #10: 50S ribosomal protein L13

+
Macromolecule #11: 50S ribosomal protein L14

+
Macromolecule #12: 50S ribosomal protein L15

+
Macromolecule #13: 50S ribosomal protein L16

+
Macromolecule #14: 50S ribosomal protein L17

+
Macromolecule #15: 50S ribosomal protein L18

+
Macromolecule #16: 50S ribosomal protein L19

+
Macromolecule #17: 50S ribosomal protein L20

+
Macromolecule #18: 50S ribosomal protein L21

+
Macromolecule #19: 50S ribosomal protein L22

+
Macromolecule #20: 50S ribosomal protein L23

+
Macromolecule #21: 50S ribosomal protein L24

+
Macromolecule #22: 50S ribosomal protein L25

+
Macromolecule #23: 50S ribosomal protein L27

+
Macromolecule #24: 50S ribosomal protein L28

+
Macromolecule #25: 50S ribosomal protein L29

+
Macromolecule #26: 50S ribosomal protein L30

+
Macromolecule #27: 50S ribosomal protein L32

+
Macromolecule #28: 50S ribosomal protein L33

+
Macromolecule #29: 50S ribosomal protein L34

+
Macromolecule #30: 50S ribosomal protein L35

+
Macromolecule #31: 50S ribosomal protein L36

+
Macromolecule #32: Ribosome-binding ATPase YchF

+
Macromolecule #33: MAGNESIUM ION

+
Macromolecule #34: ZINC ION

-
Experimental details

-
Structure determination

-
Sample preparation

-
Electron microscopy

+
Image processing

+
About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

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Aug 12, 2020. Covid-19 info

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