8VZL

DNA Ligase 1 captured with pre-step 3 ligation at the rG:C nicksite

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase 1	polymer	669	74326.7	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679)	Homo sapiens (human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B (B)	DNA/RNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*T)-R(P*G)-3')	polymer	11	3421.2	1		synthetic construct
3	C (D)	DNA (5'-D(*GP*TP*CP*CP*GP*AP*CP*CP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	18	5446.5	1		synthetic construct
4	D (C)	DNA (5'-D(P*GP*TP*CP*GP*GP*AP*C)-3')	polymer	7	2138.4	1		synthetic construct
5	E (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
6	F, G, H, I (A, B, D, C)	water	water		18.0	232	Chemie (HOH)

Sequence modifications

A: 261 - 918 (UniProt: P18858)

PDB	External Database	Details
Ala 346	Glu 346	conflict
Ala 592	Glu 592	conflict
Ala 919	-	expression tag
Ala 920	-	expression tag
Ala 921	-	expression tag
Leu 922	-	expression tag
Glu 923	-	expression tag
His 924	-	expression tag
His 925	-	expression tag
His 926	-	expression tag
His 927	-	expression tag
His 928	-	expression tag
His 929	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85332.8
Non-Polymers*	Number of molecules	1
	Total formula weight	347.2
All*	Total formula weight	85680.1

*Water molecules are not included.