8VYN

Soluble ectodomain of human cytomegalovirus (HCMV) glycoprotein B (gB) stabilized in a prefusion-like conformation in complex with 1G2 and 7H3, composite map (global and local) and model

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Envelope glycoprotein B	polymer	786	88918.6	3	UniProt (P13201) Pfam (PF12154) Pfam (PF17416) Pfam (PF17417) Pfam (PF00606) UniProt (by SIFTS) (F5HB53)	Human betaherpesvirus 5	gB
2	D, H, L (D, H, L)	7H3 Fab Heavy Chain	polymer	234	25152.1	3		Homo sapiens
3	E, I, M (E, I, M)	7H3 Fab Light Chain	polymer	216	22879.4	3		Homo sapiens
4	F, J, N (F, J, N)	1G2 Fab Heavy Chain	polymer	224	24206.2	3		Homo sapiens
5	G, K, O (G, K, O)	1G2 Fab Light Chain	polymer	214	22853.1	3		Homo sapiens
6	P, S, V (P, S, V)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
7	Q, R, T, U, W... (Q, R, T, U, W...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
8	AA, BA, CA, DA, EA... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 704 (UniProt: P13201)

PDB	External Database	Details
Leu 100	Thr 100	engineered mutation
Cys 134	Val 134	engineered mutation
Cys 222	His 222	engineered mutation
Ser 246	Cys 246	conflict
Ile 267	Ala 267	engineered mutation
Ser 457	Arg 457	conflict
Ser 460	Arg 460	conflict
Cys 653	Ile 653	engineered mutation
Cys 657	Glu 657	engineered mutation
Gly 705	-	expression tag
Ser 706	-	expression tag
Gly 707	-	expression tag
Tyr 708	-	expression tag
Ile 709	-	expression tag
Pro 710	-	expression tag
Glu 711	-	expression tag
Ala 712	-	expression tag
Pro 713	-	expression tag
Arg 714	-	expression tag
Asp 715	-	expression tag
Gly 716	-	expression tag
Gln 717	-	expression tag
Ala 718	-	expression tag
Tyr 719	-	expression tag
Val 720	-	expression tag
Arg 721	-	expression tag
Lys 722	-	expression tag
Asp 723	-	expression tag
Gly 724	-	expression tag
Glu 725	-	expression tag
Trp 726	-	expression tag
Val 727	-	expression tag
Leu 728	-	expression tag
Leu 729	-	expression tag
Ser 730	-	expression tag
Thr 731	-	expression tag
Phe 732	-	expression tag
Leu 733	-	expression tag
Gly 734	-	expression tag
Ala 735	-	expression tag
Ala 736	-	expression tag
Ala 737	-	expression tag
Ser 738	-	expression tag
Leu 739	-	expression tag
Glu 740	-	expression tag
Val 741	-	expression tag
Leu 742	-	expression tag
Phe 743	-	expression tag
Gln 744	-	expression tag
Gly 745	-	expression tag
Pro 746	-	expression tag
Gly 747	-	expression tag
His 748	-	expression tag
His 749	-	expression tag
His 750	-	expression tag
His 751	-	expression tag
His 752	-	expression tag
His 753	-	expression tag
His 754	-	expression tag
His 755	-	expression tag
Ser 756	-	expression tag
Ala 757	-	expression tag
Trp 758	-	expression tag
Ser 759	-	expression tag
His 760	-	expression tag
Pro 761	-	expression tag
Gln 762	-	expression tag
Phe 763	-	expression tag
Glu 764	-	expression tag
Lys 765	-	expression tag
Gly 766	-	expression tag
Gly 767	-	expression tag
Ala 768	-	expression tag
Ser 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Gly 772	-	expression tag
Gly 773	-	expression tag
Ser 774	-	expression tag
Gly 775	-	expression tag
Gly 776	-	expression tag
Ser 777	-	expression tag
Ala 778	-	expression tag
Trp 779	-	expression tag
Ser 780	-	expression tag
His 781	-	expression tag
Pro 782	-	expression tag
Gln 783	-	expression tag
Phe 784	-	expression tag
Glu 785	-	expression tag
Lys 786	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	552027.9
Branched	Number of molecules	9
	Total formula weight	4306.0
Non-Polymers*	Number of molecules	12
	Total formula weight	2654.5
All*	Total formula weight	558988.5

*Water molecules are not included.