8VYB

Cryo-EM structure of human core Rab3GAP1/2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Isoform 2 of Rab3 GTPase-activating protein catalytic subunit	polymer	1038	116747.2	1	UniProt (Q15042) Pfam (PF13890) Pfam (PF19533)	Homo sapiens (human)	RAB3 GTPase-activating protein 130 kDa subunit,Rab3-GAP p130,Rab3-GAP
2	B (B)	Rab3 GTPase-activating protein non-catalytic subunit	polymer	585	65121.4	1	UniProt (Q9H2M9) Pfam (PF14655)	Homo sapiens (human)	RGAP-iso,Rab3 GTPase-activating protein 150 kDa subunit,Rab3-GAP p150,Rab3-GAP150,Rab3-GAP regulatory subunit

Sequence modifications

A: 1 - 974 (UniProt: Q15042)

PDB	External Database	Details
Met -49	-	initiating methionine
Trp -48	-	expression tag
Ser -47	-	expression tag
His -46	-	expression tag
Pro -45	-	expression tag
Gln -44	-	expression tag
Phe -43	-	expression tag
Glu -42	-	expression tag
Lys -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Gly -38	-	expression tag
Ser -37	-	expression tag
Gly -36	-	expression tag
Gly -35	-	expression tag
Gly -34	-	expression tag
Ser -33	-	expression tag
Gly -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Ser -29	-	expression tag
Trp -28	-	expression tag
Ser -27	-	expression tag
His -26	-	expression tag
Pro -25	-	expression tag
Gln -24	-	expression tag
Phe -23	-	expression tag
Glu -22	-	expression tag
Lys -21	-	expression tag
Leu -20	-	expression tag
Glu -19	-	expression tag
Val -18	-	expression tag
Leu -17	-	expression tag
Phe -16	-	expression tag
Gln -15	-	expression tag
Gly -14	-	expression tag
Pro -13	-	expression tag
Arg -12	-	expression tag
Ser -11	-	expression tag
Glu -10	-	expression tag
Ala -9	-	expression tag
Arg -8	-	expression tag
Gly -7	-	expression tag
Ile -6	-	expression tag
Gln -5	-	expression tag
Arg -4	-	expression tag
Pro -3	-	expression tag
Thr -2	-	expression tag
Ser -1	-	expression tag
Thr 0	-	expression tag
Ser 975	-	expression tag
Ser 976	-	expression tag
Asp 977	-	expression tag
Thr 978	-	expression tag
Ser 979	-	expression tag
Phe 980	-	expression tag
Phe 981	-	expression tag

B: 1 - 547 (UniProt: Q9H2M9)

PDB	External Database	Details
Leu 548	-	expression tag
Arg 549	-	expression tag
Glu 550	-	expression tag
Gln 551	-	expression tag
Lys 552	-	expression tag
Leu 553	-	expression tag
Glu 554	-	expression tag
Leu 555	-	expression tag
Gly 556	-	expression tag
Gly 557	-	expression tag
Ser 558	-	expression tag
Gly 559	-	expression tag
Gly 560	-	expression tag
Arg 561	-	expression tag
Gln 562	-	expression tag
Leu 563	-	expression tag
Asp 564	-	expression tag
Tyr 565	-	expression tag
Lys 566	-	expression tag
Asp 567	-	expression tag
His 568	-	expression tag
Asp 569	-	expression tag
Gly 570	-	expression tag
Asp 571	-	expression tag
Tyr 572	-	expression tag
Lys 573	-	expression tag
Asp 574	-	expression tag
His 575	-	expression tag
Asp 576	-	expression tag
Ile 577	-	expression tag
Asp 578	-	expression tag
Tyr 579	-	expression tag
Lys 580	-	expression tag
Asp 581	-	expression tag
Asp 582	-	expression tag
Asp 583	-	expression tag
Asp 584	-	expression tag
Lys 585	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	181868.6
All*	Total formula weight	181868.6

*Water molecules are not included.