8VTA

SthK R120A in the presence of PIP2 and cAMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Transcriptional regulator, Crp/Fnr family	polymer	453	50675.0	4	UniProt (G0GA88) Pfam (PF00520) Pfam (PF07885) Pfam (PF00027)	Spirochaeta thermophila
2	E, EA, O, W (A, D, B, C)	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE	non-polymer		329.2	4	Chemie (CMP)
3	F, FA, P, X (A, D, B, C)	[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate	non-polymer		1047.1	4	Chemie (PT5)
4	AA, BA, CA, DA, G... (C, D, A, B)	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		787.1	24	Chemie (PCW)

Sequence modifications

A, B, C, D: 1 - 420 (UniProt: G0GA88)

PDB	External Database	Details
Met -15	-	expression tag
Ala -14	-	expression tag
Lys -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Gly -10	-	expression tag
Ile -9	-	expression tag
Asn -8	-	expression tag
Ser -7	-	expression tag
Asp -6	-	expression tag
Pro -5	-	expression tag
Asn -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Val 0	-	expression tag
Ala 120	Arg 120	engineered mutation
Leu 421	-	expression tag
Glu 422	-	expression tag
Ser 423	-	expression tag
Ser 424	-	expression tag
Gly 425	-	expression tag
Leu 426	-	expression tag
Val 427	-	expression tag
Pro 428	-	expression tag
Arg 429	-	expression tag
Gly 430	-	expression tag
Ser 431	-	expression tag
Val 432	-	expression tag
Lys 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	202700.1
Non-Polymers*	Number of molecules	32
	Total formula weight	24396.1
All*	Total formula weight	227096.2

*Water molecules are not included.