8VME

Crystal structure of the GSK-3/Axin complex bound to a phosphorylated beta-catenin T41A peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycogen synthase kinase-3 beta	polymer	365	41666.8	1	UniProt (Q9WV60) Pfam (PF00069)	Mus musculus (house mouse)	GSK-3 beta,Serine/threonine-protein kinase GSK3B
2	B (B)	Axin-1	polymer	24	2738.1	1	UniProt (O35625) UniProt (by SIFTS) (O15169)	Homo sapiens (human)	Axis inhibition protein 1,Protein Fused
3	C (C)	Catenin beta-1	polymer	28	2912.0	1	UniProt (P35222)	Homo sapiens (human)	Beta-catenin
4	D (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G, H, I, J, K... (A)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
7	M (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	N, O (A, B)	water	water		18.0	174	Chemie (HOH)

Sequence modifications

A: 26 - 383 (UniProt: Q9WV60)

PDB	External Database	Details
Gln 25	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag

B: 383 - 402 (UniProt: O35625)

PDB	External Database	Details
Gly 379	-	expression tag
Gly 380	-	expression tag
Ile 381	-	expression tag
Leu 382	-	expression tag

C: 35 - 61 (UniProt: P35222)

PDB	External Database	Details
Met 34	-	initiating methionine
Ala 41	Thr 41	variant

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	47316.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1051.4
All*	Total formula weight	48368.3

*Water molecules are not included.