8VHD
Crystal Structure of Human IDH1 R132Q in complex with NADPH and Isocitrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Isocitrate dehydrogenase [NADP] cytoplasmic | polymer | 430 | 48500.1 | 4 | UniProt (O75874) Pfam (PF00180) | Homo sapiens (human) | IDH,IDH1,Cytosolic NADP-isocitrate dehydrogenase,IDPc,NADP(+)-specific ICDH,Oxalosuccinate decarboxylase |
| 2 | E, I, N, Q (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
| 3 | F, J, O, T (A, B, C, D) | IODIDE ION | non-polymer | 126.9 | 4 | Chemie (IOD) | |||
| 4 | G, K, P, U (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 5 | H, M, R, S (B, C, D) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | L (C) | ISOCITRIC ACID | non-polymer | 192.1 | 1 | Chemie (ICT) | |||
| 7 | V, W, X, Y (A, B, C, D) | water | water | 18.0 | 801 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 414 (UniProt: O75874)
| PDB | External Database | Details |
|---|---|---|
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Gln 132 | Arg 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 194000.5 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 4202.0 | |
| All* | Total formula weight | 198202.5 |
