8VGO

CryoEM structure of CD20 in complex with engineered conformationally rigid Rituximab.4DS Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, H)	Rituximab.4DS Fab heavy chain	polymer	237	25345.4	2		Homo sapiens
2	B, F (B, L)	Rituximab.4DS Fab light chain	polymer	213	23106.8	2		Homo sapiens
3	C, E (G, I)	B-lymphocyte antigen CD20	polymer	278	31320.8	2	UniProt (P11836) Pfam (PF04103)	Homo sapiens (human)	B-lymphocyte surface antigen B1,Bp35,Leukocyte surface antigen Leu-16,Membrane-spanning 4-domains subfamily A member 1

Sequence modifications

G, I: 41 - 297 (UniProt: P11836)

PDB	External Database	Details
Met 38	-	initiating methionine
Gly 39	-	expression tag
Ser 40	-	expression tag
Gly 298	-	expression tag
Asn 299	-	expression tag
Ser 300	-	expression tag
Glu 301	-	expression tag
Asn 302	-	expression tag
Leu 303	-	expression tag
Tyr 304	-	expression tag
Phe 305	-	expression tag
Gln 306	-	expression tag
Gly 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	159545.9
All*	Total formula weight	159545.9

*Water molecules are not included.