8V4Y
Cryo-EM structure of singly-bound SNF2h-nucleosome complex with SNF2h at inactive SHL2 (conformation 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3.2 | polymer | 135 | 15271.9 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | Widom 601 DNA (147-mer) with 60 base pairs flanking DNA (reverse strand) | polymer | 207 | 64200.9 | 1 | synthetic construct | ||
| 6 | J (J) | Widom 601 DNA (147-mer) with 60 base pairs flanking DNA (forward strand) | polymer | 207 | 63626.5 | 1 | synthetic construct | ||
| 7 | K (W) | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | polymer | 1052 | 122089.3 | 1 | UniProt (O60264) Pfam (PF00176) Pfam (PF00271) Pfam (PF09110) Pfam (PF09111) | Homo sapiens (human) | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5,Sucrose nonfermenting protein 2 homolog,hSNF2H |
| 8 | L (W) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 9 | M (W) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | N (W) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) |
Sequence modifications
A, E: 1 - 135 (UniProt: P84233)
C, G: 1 - 129 (UniProt: P06897)
D, H: 1 - 122 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | engineered mutation |
| Ala 110 | Cys 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| Ser 123 | Ala 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 357992.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 517.5 | |
| All* | Total formula weight | 358510.4 |
