8V1V

Human DNA Ligase I F872L bound to adenylated nicked DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase 1	polymer	647	71998.3	1	UniProt (P18858)	Homo sapiens (human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B (B)	DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*C)-3')	polymer	11	3365.2	1		synthetic construct
3	C (C)	DNA (5'-D(P*GP*TP*CP*GP*GP*AP*C)-3')	polymer	7	2138.4	1		synthetic construct
4	D (D)	DNA (5'-D(*GP*TP*CP*CP*GP*AP*(OHU)P*GP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	18	5503.5	1		synthetic construct
5	E, G, H (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	F, K (A, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
7	I (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
8	J (C)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
9	L, M, N, O (A, B, C, D)	water	water		18.0	237	Chemie (HOH)

Sequence modifications

A: 262 - 904 (UniProt: P18858)

PDB	External Database	Details
Ser 258	-	expression tag
Asn 259	-	expression tag
Asp 260	-	expression tag
Met 261	-	expression tag
Leu 872	Phe 872	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	83005.4
Non-Polymers*	Number of molecules	7
	Total formula weight	1037.0
All*	Total formula weight	84042.4

*Water molecules are not included.