8UW1

Cryo-EM structure of DNMT3A1 UDR in complex with H2AK119Ub-nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2	polymer	136	15421.1	2	UniProt (P84233) In PDB	Xenopus laevis (African clawed frog)	Histone H3
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (A0A8J1LTD2) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B 1.1	polymer	126	13979.3	2	UniProt (P02281) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	K	DNA (cytosine-5)-methyltransferase 3A	polymer	76	9028.4	1	UniProt (Q9Y6K1) In PDB	Homo sapiens	Dnmt3a,Cysteine methyltransferase DNMT3A,DNA methyltransferase HsaIIIA,DNA MTase HsaIIIA,M.HsaIIIA
6	I	DNA (146-MER)	polymer	146	44824.6	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
7	J	DNA (146-MER)	polymer	146	45304.9	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Sequence modifications

D, H: -3 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	conflict

K: 159 - 228 (UniProt: Q9Y6K1)

PDB	External Database	Details
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	208966.3
All*	Total formula weight	208966.3

*Water molecules are not included.