8UT4

CryoEM structure of A/Michigan/45/2015 H1 in complex with flu HA central stem VH1-18 antibody 09-1B12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	324	36018.5	3	UniProt (A0A6J3XHU5) Pfam (PF00509) UniProt (by SIFTS) (A0A1J0CXU0) In PDB	Influenza A virus
2	B, D, F	Hemagglutinin HA2 chain	polymer	250	28359.5	3	UniProt (A0A6G7M316) Pfam (PF00509) UniProt (by SIFTS) (A0A109YUH3) In PDB	Influenza A virus
3	I	09-1B12 HC Fv	polymer	127	13967.6	1		Homo sapiens
4	H	09-1B12 LC Fv	polymer	109	12121.5	1		Homo sapiens
5	A, B, C, D, E...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

B, D, F: -3 - 177 (UniProt: A0A6G7M316)

PDB	External Database	Details
Gly 178	-	expression tag
Ala 179	-	expression tag
Leu 180	-	expression tag
Glu 181	-	expression tag
Val 182	-	expression tag
Leu 183	-	expression tag
Phe 184	-	expression tag
Gln 185	-	expression tag
Gly 186	-	expression tag
Pro 187	-	expression tag
Gly 188	-	expression tag
Ser 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
Leu 198	-	expression tag
Gly 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Gly 202	-	expression tag
Tyr 203	-	expression tag
Ile 204	-	expression tag
Pro 205	-	expression tag
Glu 206	-	expression tag
Ala 207	-	expression tag
Pro 208	-	expression tag
Arg 209	-	expression tag
Asp 210	-	expression tag
Gly 211	-	expression tag
Gln 212	-	expression tag
Ala 213	-	expression tag
Tyr 214	-	expression tag
Val 215	-	expression tag
Arg 216	-	expression tag
Lys 217	-	expression tag
Asp 218	-	expression tag
Gly 219	-	expression tag
Glu 220	-	expression tag
Trp 221	-	expression tag
Val 222	-	expression tag
Leu 223	-	expression tag
Leu 224	-	expression tag
Ser 225	-	expression tag
Thr 226	-	expression tag
Phe 227	-	expression tag
Leu 228	-	expression tag
Gly 229	-	expression tag
Ser 230	-	expression tag
Gly 231	-	expression tag
Gly 232	-	expression tag
Gly 233	-	expression tag
Leu 234	-	expression tag
Asn 235	-	expression tag
Asp 236	-	expression tag
Ile 237	-	expression tag
Phe 238	-	expression tag
Glu 239	-	expression tag
Ala 240	-	expression tag
Gln 241	-	expression tag
Lys 242	-	expression tag
Ile 243	-	expression tag
Glu 244	-	expression tag
Trp 245	-	expression tag
His 246	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	219223.0
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	223204.8

*Water molecules are not included.