8UQC

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 20-residue linker (crystallization condition 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase RNF168,Ubiquitin-conjugating enzyme E2 D3,Histone H2B type 2-E,Histone H2A type 1-B/E	polymer	455	50040.3	1	UniProt (Q8IYW5) UniProt (P61077) UniProt (Q16778) UniProt (P04908) Pfam (PF14447) Pfam (PF00179) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)
2	B, C (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D (A)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 1 - 94 (UniProt: Q8IYW5)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Ser 95	-	linker
Gly 96	-	linker
Ser 97	-	linker
Gly 98	-	linker
Ser 99	-	linker
Gly 100	-	linker
Ser 101	-	linker

A: 1002 - 1147 (UniProt: P61077)

PDB	External Database	Details
Lys 1085	Cys 85	engineered mutation
Gly 1148	-	linker
Gly 1149	-	linker
Ser 1150	-	linker
Gly 1151	-	linker
Gly 1152	-	linker
Gly 1153	-	linker
Ser 1154	-	linker
Gly 1155	-	linker
Gly 1156	-	linker
Ser 1157	-	linker
Gly 1158	-	linker
Gly 1159	-	linker
Ser 1160	-	linker
Gly 1161	-	linker
Gly 1162	-	linker
Ser 1163	-	linker
Gly 1164	-	linker
Ser 1165	-	linker
Gly 1166	-	linker
Ser 1167	-	linker

A: 2033 - 2123 (UniProt: Q16778)

PDB	External Database	Details
Ser 3011	-	linker

A: 3012 - 3105 (UniProt: P04908)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50040.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	50171.1

*Water molecules are not included.