8UPC

Structure of atypical asparaginase from Rhodospirillum rubrum (mutant K158M)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Asparaginase	polymer	185	19803.5	2	UniProt (Q2RMX1) Pfam (PF00710)	Rhodospirillum rubrum ATCC 11170
2	C, F (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H (A, B)	water	water		18.0	341	Chemie (HOH)

Sequence modifications

A, B: 1 - 172 (UniProt: Q2RMX1)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Met 158	Lys 158	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39607.0
Non-Polymers*	Number of molecules	4
	Total formula weight	255.1
All*	Total formula weight	39862.1

*Water molecules are not included.