8UOW

Structure of atypical asparaginase from Rhodospirillum rubrum (mutant Y21A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Asparaginase	polymer	185	19709.4	4	UniProt (Q2RMX1) Pfam (PF00710)	Rhodospirillum rubrum ATCC 11170
2	E, F, G, H (A, B, C, D)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	4	Chemie (EPE)
3	I, J, K, L (A, B, C, D)	water	water		18.0	616	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 172 (UniProt: Q2RMX1)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Ala 21	Tyr 21	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78837.6
Non-Polymers*	Number of molecules	4
	Total formula weight	953.2
All*	Total formula weight	79790.8

*Water molecules are not included.