8UNI

LSD1-CoREST with N-formyl-FAD in complex with H3K4M histone tail

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lysine-specific histone demethylase 1A	polymer	871	95051.6	1	UniProt (O60341) Pfam (PF04433) Pfam (PF01593)	Homo sapiens (human)	BRAF35-HDAC complex protein BHC110,Flavin-containing amine oxidase domain-containing protein 2,[histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A
2	B (B)	REST corepressor 1	polymer	144	16459.7	1	UniProt (Q9UKL0) Pfam (PF20878) Pfam (PF00249)	Homo sapiens (human)	Protein CoREST
3	C (C)	Histone H3 (Fragment)	polymer	21	2263.7	1	UniProt (V9H1G0)	Homo sapiens (human)
4	D (A)	[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-[5-methanoyl-7,8-dimethyl-2,4-bis(oxidanylidene)-1H-benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate	non-polymer		815.6	1	Chemie (HUF)

Sequence modifications

A: 1 - 852 (UniProt: O60341)

PDB	External Database	Details
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: 305 - 440 (UniProt: Q9UKL0)

PDB	External Database	Details
Gly 297	-	expression tag
Pro 298	-	expression tag
Leu 299	-	expression tag
Gly 300	-	expression tag
Ser 301	-	expression tag
Pro 302	-	expression tag
Glu 303	-	expression tag
Phe 304	-	expression tag

C: 1 - 21 (UniProt: V9H1G0)

PDB	External Database	Details
Met 4	Lys 5	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	113775.0
Non-Polymers*	Number of molecules	1
	Total formula weight	815.6
All*	Total formula weight	114590.6

*Water molecules are not included.