8UKW

Crystal structure the extracellular region of the epidermal growth factor receptor variant III (EGFRvIII) at pH 5.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Epidermal growth factor receptor	polymer	357	39210.5	2	UniProt (P00533) Pfam (PF01030) Pfam (PF14843)	Homo sapiens (human)	Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1
2	C (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
3	D, E (D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	F, G, H, I (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	J, K (A, B)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

A, B: 268 - 273 (UniProt: P00533)

PDB	External Database	Details
Gly 273	Val 30	engineered mutation

A, B: 274 - 618 (UniProt: P00533)

PDB	External Database	Details
Ser 283	Cys 307	engineered mutation
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag
His 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78420.9
Branched	Number of molecules	3
	Total formula weight	1435.3
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	80741.1

*Water molecules are not included.