8UKW

Crystal structure the extracellular region of the epidermal growth factor receptor variant III (EGFRvIII) at pH 5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-10
Detector	MAR CCD 300 mm
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	48.818, 88.294, 99.751
Unit cell angles	90.00, 95.25, 90.00

Refinement procedure

Resolution	45.330 - 2.390
R-factor	0.2163
Rwork	0.215
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.066	0.705
Rmeas	0.079	0.835
Rpim	0.042	0.443
Total number of observations	115842	12278
Number of reflections	33254	3486
<I/σ(I)>	11.7	1.8
Completeness [%]	99.3
Redundancy	3.5	3.5
CC(1/2)	0.998	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	7 mg/ml protein, 27.5% PEG 3350, 50 mM Na Citrate pH 5.0