8UKV

Crystal structure of nanobody/VHH domain of 34E5 in complex with the extracellular region of the epidermal growth factor variant III (EGFRvIII)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Epidermal growth factor receptor	polymer	357	39226.5	2	UniProt (P00533) Pfam (PF01030) Pfam (PF14843)	Homo sapiens (human)	Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1
2	C, D (C, D)	Nanobody/VHH domain 34E5	polymer	141	15744.4	2		Lama glama (llama)
3	E, F, G (E, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	H (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, B: 268 - 273 (UniProt: P00533)

PDB	External Database	Details
Gly 273	Val 30	engineered mutation

A, B: 274 - 618 (UniProt: P00533)

PDB	External Database	Details
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag
His 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	109941.8
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	111436.2

*Water molecules are not included.