8UKV

Crystal structure of nanobody/VHH domain of 34E5 in complex with the extracellular region of the epidermal growth factor variant III (EGFRvIII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-30
Detector	RAYONIX MX-300
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	66.291, 80.361, 91.841
Unit cell angles	106.92, 103.50, 104.56

Refinement procedure

Resolution	46.550 - 2.940
R-factor	0.2168
Rwork	0.215
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.310	2.870
High resolution limit [Å]	2.755	2.760
Rmerge	0.098	1.954
Rmeas	0.114	2.266
Rpim	0.058	1.143
Total number of observations		13150
Number of reflections	40641	3379
<I/σ(I)>	9	0.7
Completeness [%]	94.3
Redundancy	3.9	3.9
CC(1/2)	0.994	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	70-90 micromolar protein, 15-20% PEG 3350, 100 mM ammonium phosphate, pH 7.4, +20% PEG400 for cryo