8UK9

Structure of T4 Bacteriophage clamp loader mutant D110C bound to the T4 clamp, primer-template DNA, and ATP analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, Q	Sliding-clamp-loader small subunit	polymer	187	21391.7	2	UniProt (P04527) Pfam (PF16790) In PDB	Tequatrovirus T4	Clamp loader gp62 subunit,Gene product 62,gp62
2	B, C, D, E, K...	Sliding-clamp-loader large subunit	polymer	320	35909.4	8	UniProt (P04526) Pfam (PF00004) Pfam (PF21328) Pfam (PF21429) In PDB	Tequatrovirus T4
3	F, G, H, R, S...	Sliding clamp	polymer	228	24881.2	6	UniProt (P04525) Pfam (PF02916) Pfam (PF09116) In PDB	Tequatrovirus T4	GP45
4	I, O	DNA template	polymer	24	7347.7	2		Tequatrovirus T4
5	J, P	DNA primer	polymer	20	6136.0	2		Tequatrovirus T4
6	D, K, L, M, B...	ALUMINUM FLUORIDE	non-polymer		84.0	6	Chemie (AF3)
7	D, E, K, L, M...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	8	Chemie (ADP)
8	D, E, K, L, M...	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)

Sequence modifications

B, C, D, E, K, L, M, N: 1 - 319 (UniProt: P04526)

PDB	External Database	Details
Ser 0	-	expression tag
Cys 110	Asp 110	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	506313.3
Non-Polymers*	Number of molecules	22
	Total formula weight	4115.9
All*	Total formula weight	510429.2

*Water molecules are not included.