8UK9

Structure of T4 Bacteriophage clamp loader mutant D110C bound to the T4 clamp, primer-template DNA, and ATP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	95.243, 231.991, 264.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.130 - 3.100
R-factor	0.253
Rwork	0.253
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.664
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	3.211
High resolution limit [Å]	3.100	3.100
Rmerge	0.057	1.571
Rmeas	0.081	2.222
Rpim	0.057	1.571
Number of reflections	106987	470
<I/σ(I)>	5.8
Completeness [%]	71.4
Redundancy	2	2
CC(1/2)	0.999	0.169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	298	0.1M MES pH 6.5, 9% PEG MME 5000, 6% 1-Propanol