8UHB

Cryo-EM Structure of the Ro5256390-bound hTA1-Gs heterotrimer signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Soluble cytochrome b562,Beta-2 adrenergic receptor,Trace amine-associated receptor 1	polymer	524	60019.8	1	UniProt (P0ABE7) UniProt (P07550) UniProt (Q96RJ0) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45803.6	1	UniProt (P63095) Pfam (PF00503)	Rattus norvegicus (Norway rat)
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (N)	Nanobody 35	polymer	134	14714.3	1		Lama glama
6	F (A)	(2R,4S)-4-[(2S)-2-phenylbutyl]-1,3-oxazolidin-2-amine	non-polymer		218.3	1	Chemie (WV8)

Sequence modifications

A: -135 - -35 (UniProt: P0ABE7)

PDB	External Database	Details
Met -184	-	expression tag
Lys -183	-	expression tag
Thr -182	-	expression tag
Ile -181	-	expression tag
Ile -180	-	expression tag
Ala -179	-	expression tag
Leu -178	-	expression tag
Ser -177	-	expression tag
Tyr -176	-	expression tag
Ile -175	-	expression tag
Phe -174	-	expression tag
Cys -173	-	expression tag
Leu -172	-	expression tag
Val -171	-	expression tag
Phe -170	-	expression tag
Ala -169	-	expression tag
Asp -168	-	expression tag
Tyr -167	-	expression tag
Lys -166	-	expression tag
Asp -165	-	expression tag
Asp -164	-	expression tag
Asp -163	-	expression tag
Asp -162	-	expression tag
Ala -161	-	expression tag
Lys -160	-	expression tag
Leu -159	-	expression tag
Gln -158	-	expression tag
Thr -157	-	expression tag
Met -156	-	expression tag
His -155	-	expression tag
His -154	-	expression tag
His -153	-	expression tag
His -152	-	expression tag
His -151	-	expression tag
His -150	-	expression tag
His -149	-	expression tag
His -148	-	expression tag
His -147	-	expression tag
His -146	-	expression tag
Glu -145	-	expression tag
Asn -144	-	expression tag
Leu -143	-	expression tag
Tyr -142	-	expression tag
Phe -141	-	expression tag
Gln -140	-	expression tag
Gly -139	-	expression tag
Gly -138	-	expression tag
Thr -137	-	expression tag
Thr -136	-	expression tag
Trp -129	Met 29	engineered mutation
Ile -34	-	linker
Gln -33	-	linker
Lys -32	-	linker
Tyr -31	-	linker
Leu -30	-	linker

A: -29 - 0 (UniProt: P07550)

PDB	External Database	Details
Arg -14	Gly 16	variant
Gln -3	Glu 27	variant

A: 1 - 339 (UniProt: Q96RJ0)

PDB	External Database	Details
Trp 112	Phe 112	engineered mutation

B: 7 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Glu 3	-	expression tag
Leu 4	-	expression tag
Asp 5	-	expression tag
Gln 6	-	expression tag

C: 1 - 394 (UniProt: P63095)

PDB	External Database	Details
Glu 189	Asp 189	engineered mutation
Met 191	Val 191	engineered mutation
Thr 193	Ser 193	engineered mutation
Glu 194	Asp 194	engineered mutation
Ala 226	Gly 226	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation
Ser 366	Ala 366	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	167817.0
Non-Polymers*	Number of molecules	1
	Total formula weight	218.3
All*	Total formula weight	168035.3

*Water molecules are not included.