8UH3

Serotonin 1E receptor (5-HT1eR)-Gi1 Complex bound with Setiptiline

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (D)	5-hydroxytryptamine receptor 1E	polymer	365	41801.1	1	UniProt (P28566) Pfam (PF00001)	Homo sapiens (human)
4	D (E)	ScFv16	polymer	259	27784.9	1		Mus musculus
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F, G, H, I (D)	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR)
7	J (D)	Setiptiline	non-polymer		261.4	1	Chemie (WOQ)
8	K (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 365 (UniProt: P28566)

PDB	External Database	Details
Trp 111	Leu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	157280.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1808.0
All*	Total formula weight	159088.2

*Water molecules are not included.