8UGY

Serotonin 1E receptor (5-HT1eR)-Gi1 Complex bound with Mianserin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	R	5-hydroxytryptamine receptor 1E	polymer	339	38822.6	1	UniProt (P28566) Pfam (PF00001) In PDB	Homo sapiens (human)	5-HT-1E,5-HT1E,S31,Serotonin receptor 1E
5	R	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)
6	R	Mianserin	non-polymer		264.4	1	Chemie (WRU)
7		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 7 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Glu 3	-	expression tag
Leu 4	-	expression tag
Asp 5	-	expression tag
Gln 6	-	expression tag

R: 20 - 358 (UniProt: P28566)

PDB	External Database	Details
Trp 111	Leu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	126515.9
Non-Polymers*	Number of molecules	6
	Total formula weight	2197.6
All*	Total formula weight	128713.5

*Water molecules are not included.