8U8U

Cryo-EM Structure of Cognate Substrate ATP Bound in the Entry Site (ES) of Human Mitochondrial Transcription Elongation Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transcription elongation factor, mitochondrial	polymer	232	27262.4	2	UniProt (Q96QE5) In PDB	Homo sapiens (human)
2	E	DNA-directed RNA polymerase, mitochondrial	polymer	1119	127150.1	1	UniProt (O00411) In PDB	Homo sapiens (human)
3	N	Non-Template Strand DNA (NT27mt)	polymer	34	10483.8	1		synthetic construct
4	R	RNA (RNA14mt)	polymer	14	4493.7	1		synthetic construct
5	T	Template Strand DNA (TS31mt)	polymer	34	10419.6	1		synthetic construct
6	E	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	1	Chemie (APC)

Sequence modifications

A, B: 146 - 360 (UniProt: Q96QE5)

PDB	External Database	Details
Met 139	-	initiating methionine
Asp 140	-	expression tag
Glu 141	-	expression tag
Asn 142	-	expression tag
Ala 143	-	expression tag
Lys 144	-	expression tag
Glu 145	-	expression tag
Leu 361	-	expression tag
Glu 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

E: 120 - 1230 (UniProt: O00411)

PDB	External Database	Details
Met 112	-	expression tag
Gly 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
Ala 555	Glu 555	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	207072.0
Non-Polymers*	Number of molecules	1
	Total formula weight	505.2
All*	Total formula weight	207577.2

*Water molecules are not included.