8U8F

GPR3 Orphan G-coupled Protein Receptor in complex with Dominant Negative Gs.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37413.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	R	G-protein coupled receptor 3	polymer	390	41788.9	1	UniProt (P46089) Pfam (PF00001) In PDB	Homo sapiens (human)
5	R	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict
Ser 366	Ala 366	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gln 1	-	expression tag

R: 2 - 330 (UniProt: P46089)

PDB	External Database	Details
Asn -33	-	expression tag
Ser -32	-	expression tag
Thr -31	-	expression tag
Met -30	-	expression tag
Lys -29	-	expression tag
Thr -28	-	expression tag
Ile -27	-	expression tag
Ile -26	-	expression tag
Ala -25	-	expression tag
Leu -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Ile -21	-	expression tag
Phe -20	-	expression tag
Cys -19	-	expression tag
Leu -18	-	expression tag
Val -17	-	expression tag
Phe -16	-	expression tag
Ala -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Ala 1	-	expression tag
Pro 331	-	expression tag
Ala 332	-	expression tag
Gly 333	-	expression tag
Leu 334	-	expression tag
Glu 335	-	expression tag
Val 336	-	expression tag
Leu 337	-	expression tag
Phe 338	-	expression tag
Gln 339	-	expression tag
Gly 340	-	expression tag
Pro 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
Ala 350	-	expression tag
Ala 351	-	expression tag
Ala 352	-	expression tag
Phe 353	-	expression tag
Glu 354	-	expression tag
Ser 355	-	expression tag
Arg 356	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	131277.5
Non-Polymers*	Number of molecules	1
	Total formula weight	256.4
All*	Total formula weight	131533.9

*Water molecules are not included.