8U72

Cryo-EM structure of the SPARTA oligomer with guide RNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	U, M, S, X	RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*A)-3')	polymer	21	6812.0	4		synthetic construct
2	V, N, T, Y	DNA (5'-D(P*TP*AP*TP*AP*CP*AP*AP*CP*CP*TP*AP*CP*TP*AP*CP*CP*TP*C)-3')	polymer	45	13703.8	4		synthetic construct
3	H, D, B, F	TIR domain-containing protein	polymer	450	53256.7	4	UniProt (A0A1I7NFG5) Pfam (PF13676) In PDB	Thermoflavifilum thermophilum	TIR-APAZ
4	I, E, C, G	Piwi domain-containing protein	polymer	507	58304.8	4	UniProt (A0A1I7NFD7) In PDB	Thermoflavifilum thermophilum	pAgo
5	I, E, C, G	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6		water	water		18.0	57	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	528309.9
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	528407.1

*Water molecules are not included.