8U4V
Cryo-EM structure of human claudin-4 complex with Clostridium perfringens enterotoxin C-terminal domain, sFab COP-1, and Nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Claudin-4 | polymer | 214 | 22613.9 | 1 | UniProt (O14493) Pfam (PF00822) | Homo sapiens (human) | Clostridium perfringens enterotoxin receptor,CPE-receptor,Williams-Beuren syndrome chromosomal region 8 protein |
| 2 | B (B) | Heat-labile enterotoxin B chain | polymer | 125 | 14044.6 | 1 | UniProt (P01558) | Clostridium perfringens | |
| 3 | C (L) | COP-1 sFab Light Chain | polymer | 215 | 23258.8 | 1 | Homo sapiens (human) | ||
| 4 | D (H) | COP-1 sFab Heavy Chain | polymer | 261 | 28064.5 | 1 | Homo sapiens (human) | ||
| 5 | E (K) | Anti-fab nanobody | polymer | 121 | 13175.4 | 1 | synthetic construct | ||
| 6 | F (H) | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (AV0) | |||
| 7 | G, H (A, B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 1 - 209 (UniProt: O14493)
B: 198 - 319 (UniProt: P01558)
| PDB | External Database | Details |
|---|---|---|
| Gly 210 | - | expression tag |
| Leu 211 | - | expression tag |
| Val 212 | - | expression tag |
| Pro 213 | - | expression tag |
| Arg 214 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 195 | - | expression tag |
| Ser 196 | - | expression tag |
| Asp 197 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 101157.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 1005.2 | |
| All* | Total formula weight | 102162.4 |
