8U1G

Prefusion-stabilized SARS-CoV-2 S2 subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Spike protein S2	polymer	530	58310.0	2	UniProt (P0DTC2) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2 COVID-19 virus)
2	C (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, B: 697 - 1141 (UniProt: P0DTC2)

PDB	External Database	Details
Met 671	-	initiating methionine
Arg 672	-	expression tag
Pro 673	-	expression tag
Thr 674	-	expression tag
Trp 675	-	expression tag
Ala 676	-	expression tag
Trp 677	-	expression tag
Trp 678	-	expression tag
Leu 679	-	expression tag
Phe 680	-	expression tag
Leu 681	-	expression tag
Val 682	-	expression tag
Leu 683	-	expression tag
Leu 684	-	expression tag
Leu 685	-	expression tag
Ala 686	-	expression tag
Leu 687	-	expression tag
Trp 688	-	expression tag
Ala 689	-	expression tag
Pro 690	-	expression tag
Ala 691	-	expression tag
Arg 692	-	expression tag
Gly 693	-	expression tag
Ala 694	-	expression tag
Ser 695	-	expression tag
Gln 696	-	expression tag
Cys 704	Ser 704	engineered mutation
Cys 790	Lys 790	engineered mutation
Pro 817	Phe 817	engineered mutation
Pro 892	Ala 892	engineered mutation
Pro 899	Ala 899	engineered mutation
Pro 942	Ala 942	engineered mutation
Glu 957	Gln 957	engineered mutation
Pro 986	Lys 986	engineered mutation
Pro 987	Val 987	engineered mutation
Gly 1142	-	expression tag
Ser 1143	-	expression tag
Gly 1144	-	expression tag
Tyr 1145	-	expression tag
Ile 1146	-	expression tag
Pro 1147	-	expression tag
Glu 1148	-	expression tag
Ala 1149	-	expression tag
Pro 1150	-	expression tag
Arg 1151	-	expression tag
Asp 1152	-	expression tag
Gly 1153	-	expression tag
Gln 1154	-	expression tag
Ala 1155	-	expression tag
Tyr 1156	-	expression tag
Val 1157	-	expression tag
Arg 1158	-	expression tag
Lys 1159	-	expression tag
Asp 1160	-	expression tag
Gly 1161	-	expression tag
Glu 1162	-	expression tag
Trp 1163	-	expression tag
Val 1164	-	expression tag
Leu 1165	-	expression tag
Leu 1166	-	expression tag
Ser 1167	-	expression tag
Thr 1168	-	expression tag
Phe 1169	-	expression tag
Leu 1170	-	expression tag
Gly 1171	-	expression tag
Arg 1172	-	expression tag
Ser 1173	-	expression tag
Leu 1174	-	expression tag
Glu 1175	-	expression tag
Val 1176	-	expression tag
Leu 1177	-	expression tag
Phe 1178	-	expression tag
Gln 1179	-	expression tag
Gly 1180	-	expression tag
Pro 1181	-	expression tag
Gly 1182	-	expression tag
His 1183	-	expression tag
His 1184	-	expression tag
His 1185	-	expression tag
His 1186	-	expression tag
His 1187	-	expression tag
His 1188	-	expression tag
His 1189	-	expression tag
His 1190	-	expression tag
Ser 1191	-	expression tag
Ala 1192	-	expression tag
Trp 1193	-	expression tag
Ser 1194	-	expression tag
His 1195	-	expression tag
Pro 1196	-	expression tag
Gln 1197	-	expression tag
Phe 1198	-	expression tag
Glu 1199	-	expression tag
Lys 1200	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	116620.1
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	116841.3

*Water molecules are not included.