8U1G

Prefusion-stabilized SARS-CoV-2 S2 subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	H 3
Unit cell lengths	78.990, 78.990, 479.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.720 - 3.200
R-factor	0.2357
Rwork	0.226
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.561
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.920	3.420
High resolution limit [Å]	3.200	3.200
Number of reflections	18384	3362
<I/σ(I)>	3.2	1.8
Completeness [%]	99.7
Redundancy	4.4
CC(1/2)	0.850	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES pH 7.5, 8% (v/v) ethylene glycol and 10% (v/v) PEG 8000