8U0O
Synaptic complex of human DNA polymerase Lambda DL variant engaged on a DNA double-strand break containing an unpaired 3' primer terminus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 341 | 37938.4 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B (T) | DNA (5'-D(*AP*CP*GP*CP*GP*GP*CP*A)-3') | polymer | 8 | 2436.6 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(P*GP*CP*CP*GP*CP*GP*TP*A)-3') | polymer | 8 | 2427.6 | 1 | synthetic construct | ||
| 4 | D (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | E (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 6 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | G, H (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 8 | I, J, K (A, T, P) | water | water | 18.0 | 84 | Chemie (HOH) |
Sequence modifications
A: 234 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Gly 230 | - | expression tag |
| Ser 231 | - | expression tag |
| Ala 232 | - | expression tag |
| Ala 233 | - | expression tag |
| Lys 463 | Ser 463 | engineered mutation |
| Gly 464 | Gln 464 | engineered mutation |
| - | Glu 466 | deletion |
| - | Asn 467 | deletion |
| - | Gly 468 | deletion |
| - | Gln 469 | deletion |
| - | Gln 470 | deletion |
| Thr 471 | Gln 471 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 42802.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 277.5 | |
| All* | Total formula weight | 43080.2 |
