8U02

CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	D(2) dopamine receptor	polymer	443	50685.4	1	UniProt (P14416) Pfam (PF00001)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40100.4	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
3	C (A)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (R)	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP)

Sequence modifications

B: 1 - 354 (UniProt: P09471)

PDB	External Database	Details
Glu 46	Lys 46	engineered mutation

A: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	138065.0
Non-Polymers*	Number of molecules	1
	Total formula weight	153.2
All*	Total formula weight	138218.2

*Water molecules are not included.