8TYC

Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Glycoprotein G1	polymer	259	29010.4	3	UniProt (P08669) Pfam (PF00798)	Lassa virus
2	D (H)	Polyclonal antibody Base-1 heavy chain	polymer	108	9209.3	1		Oryctolagus cuniculus (rabbit)
3	E (L)	Polyclonal antibody Base-1 light chain	polymer	107	9124.2	1		Oryctolagus cuniculus (rabbit)
4	F, G, H (a, b, c)	Glycoprotein G2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein	polymer	406	44765.0	3	UniProt (P08669) UniProt (Q9WXS1) Pfam (PF00798) Pfam (PF01081)	Lassa virus	GP2
5	BA, DA, GA, I, IA... (Y, d, g, D, i...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	15	In PDB GlyTouCan (G42666HT)
6	AA, EA, J, Q, R... (X, e, E, N, O...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	7	In PDB GlyTouCan (G15407YE)
7	T (Q)	alpha-L-fucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G80587NA)
8	Z (W)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
9	CA, FA, HA (Z, f, h)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G56014GC)
10	JA, KA, LA, MA, NA... (C, a, b, c)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 259 (UniProt: P08669)

PDB	External Database	Details
Cys 207	Arg 207	conflict

a, b, c: 260 - 423 (UniProt: P08669)

PDB	External Database	Details
Pro 329	Glu 329	conflict
Cys 360	Gly 360	conflict
Gly 424	-	linker
Gly 425	-	linker
Ser 426	-	linker
Gly 427	-	linker
Gly 428	-	linker
Ser 429	-	linker
Gly 430	-	linker
Gly 431	-	linker
Ser 432	-	linker
Gly 433	-	linker
Gly 434	-	linker
Ser 435	-	linker
Gly 436	-	linker
Gly 437	-	linker
Ser 438	-	linker
Glu 439	-	linker
Lys 440	-	linker
Ala 441	-	linker
Ala 442	-	linker
Lys 443	-	linker
Ala 444	-	linker
Glu 445	-	linker
Glu 446	-	linker
Ala 447	-	linker
Ala 448	-	linker
Arg 449	-	linker

a, b, c: 450 - 653 (UniProt: Q9WXS1)

PDB	External Database	Details
Ile 473	Lys 25	conflict
Val 477	Leu 29	conflict
Ala 481	Glu 33	conflict
Ala 502	Glu 54	conflict
Val 505	Phe 57	conflict
Asp 574	Thr 126	conflict
Glu 587	Gln 139	conflict
Asp 608	Asn 160	conflict
Asp 617	Lys 169	conflict
Asp 627	Ser 179	conflict
Glu 631	Lys 183	conflict
Asp 633	Thr 185	conflict
Glu 643	Ala 195	conflict
Gly 654	-	expression tag
Ser 655	-	expression tag
Leu 656	-	expression tag
Glu 657	-	expression tag
Trp 658	-	expression tag
Ser 659	-	expression tag
His 660	-	expression tag
Pro 661	-	expression tag
Gln 662	-	expression tag
Phe 663	-	expression tag
Glu 664	-	expression tag
Lys 665	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	239659.8
Branched	Number of molecules	27
	Total formula weight	14628.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	255615.6

*Water molecules are not included.