8TWA
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-PolE-DNA complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Chromosome transmission fidelity protein 18 | polymer | 26 | 3171.6 | 1 | UniProt (P49956) | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | B (P) | Primer DNA | polymer | 9 | 2737.8 | 1 | synthetic construct | ||
| 3 | C (T) | Template DNA | polymer | 15 | 4568.0 | 1 | synthetic construct | ||
| 4 | D (E) | DNA polymerase epsilon catalytic subunit A | polymer | 2222 | 255992.5 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF22912) | Saccharomyces cerevisiae (brewer's yeast) | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
| 5 | E (D) | Chromosome transmission fidelity protein 8 | polymer | 132 | 15058.5 | 1 | UniProt (P38877) Pfam (PF09696) | Saccharomyces cerevisiae (brewer's yeast) | |
| 6 | F (B) | Sister chromatid cohesion protein DCC1 | polymer | 380 | 44133.8 | 1 | UniProt (P25559) Pfam (PF09724) | Saccharomyces cerevisiae (brewer's yeast) | Defective in sister chromatid cohesion protein 1 |
| 7 | G (4) | Replication factor C subunit 4 | polymer | 319 | 35782.5 | 1 | UniProt (P40339) Pfam (PF00004) Pfam (PF08542) | Saccharomyces cerevisiae (brewer's yeast) | |
| 8 | H (3) | Replication factor C subunit 3 | polymer | 327 | 36818.9 | 1 | UniProt (P38629) Pfam (PF00004) Pfam (PF08542) | Saccharomyces cerevisiae (brewer's yeast) | |
| 9 | I (2) | Replication factor C subunit 2 | polymer | 340 | 38256.6 | 1 | UniProt (P40348) Pfam (PF00004) Pfam (PF21960) Pfam (PF08542) | Saccharomyces cerevisiae (brewer's yeast) | |
| 10 | J (5) | Replication factor C subunit 5 | polymer | 354 | 39993.6 | 1 | UniProt (P38251) Pfam (PF21960) Pfam (PF22534) Pfam (PF13177) | Saccharomyces cerevisiae (brewer's yeast) | |
| 11 | K (1) | Chromosome transmission fidelity protein 18 | polymer | 741 | 84487.2 | 1 | UniProt (P49956) Pfam (PF00004) | Saccharomyces cerevisiae (brewer's yeast) | |
| 12 | L, M, N (X, Y, Z) | Proliferating cell nuclear antigen | polymer | 258 | 28944.1 | 3 | UniProt (P15873) Pfam (PF00705) Pfam (PF02747) | Saccharomyces cerevisiae (brewer's yeast) | PCNA |
| 13 | O (E) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 14 | P, R, T (4, 3, 2) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 3 | Chemie (AGS) | |||
| 15 | Q, S, U (4, 3, 2) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 16 | V (5) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
5: 4 - 353 (UniProt: P38251)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ser 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Asp 354 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 647833.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2421.5 | |
| All* | Total formula weight | 650254.6 |
