8TW4

TCR in nanodisc ND-I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	TCR alpha	polymer	274	30388.2	1		Homo sapiens (human)
2	B	T cell receptor beta variable 6-5, T cell receptor beta chain MC.7.G5, MCHERRY fusion protein	polymer	556	62042.9	1	UniProt (A0A0K0K1A5) UniProt (P0DTU4) UniProt (A0A4D6FVK6) In PDB	Homo sapiens (human)	TR beta chain TRBV25-1*01J2S3*01C2*01,MC.7.G5 TRB
3	E, F	T-cell surface glycoprotein CD3 epsilon chain	polymer	207	23174.2	2	UniProt (P07766) In PDB	Homo sapiens (human)
4	D	T-cell surface glycoprotein CD3 delta chain	polymer	171	18949.5	1	UniProt (P04234) In PDB	Homo sapiens (human)	T-cell receptor T3 delta chain
5	G	T-cell surface glycoprotein CD3 gamma chain	polymer	190	21598.6	1	UniProt (P09693) In PDB	Homo sapiens (human)	T-cell receptor T3 gamma chain
6	X, Y	T-cell surface glycoprotein CD3 zeta chain,RNA-directed RNA polymerase L	polymer	412	46650.9	2	UniProt (P20963) UniProt (A0A5P9VSM8) In PDB	Homo sapiens (human)	T-cell receptor T3 zeta chain,Large structural protein,Replicase,Transcriptase
7	C, H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
8	I	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
9	A, G	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
10	B, Y	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

B: 1 - 114 (UniProt: A0A0K0K1A5)

PDB	External Database	Details
Val 115	-	linker
Gly 116	-	linker
Asn 117	-	linker
Thr 118	-	linker
Gly 119	-	linker
Glu 120	-	linker
Leu 121	-	linker
Phe 122	-	linker
Phe 123	-	linker
Gly 124	-	linker
Glu 125	-	linker
Gly 126	-	linker
Ser 127	-	linker

B: 128 - 311 (UniProt: P0DTU4)

PDB	External Database	Details
Ala 312	-	linker
Ser 313	-	linker
Leu 314	-	linker
Glu 315	-	linker
Val 316	-	linker
Leu 317	-	linker
Phe 318	-	linker
Gln 319	-	linker
Gly 320	-	linker
Pro 321	-	linker

B: 322 - 556 (UniProt: A0A4D6FVK6)
G: 1 - 182 (UniProt: P09693)

PDB	External Database	Details
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag

X, Y: 1 - 164 (UniProt: P20963)

PDB	External Database	Details
Ala 165	-	linker
Ser 166	-	linker
Leu 167	-	linker
Glu 168	-	linker
Val 169	-	linker
Leu 170	-	linker
Phe 171	-	linker
Gln 172	-	linker
Gly 173	-	linker
Pro 174	-	linker

X, Y: 175 - 412 (UniProt: A0A5P9VSM8)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	272629.5
Branched	Number of molecules	3
	Total formula weight	1435.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1436.9
All*	Total formula weight	275501.7

*Water molecules are not included.