8TU9

Cryo-EM structure of HGSNAT-acetyl-CoA complex at pH 7.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Enhanced green fluorescent protein,Heparan-alpha-glucosaminide N-acetyltransferase,Isoform 2 of Heparan-alpha-glucosaminide N-acetyltransferase	polymer	903	100817.2	2	UniProt (A0A9X4KGN5) UniProt (Q68CP4) Pfam (PF01353) Pfam (PF07786) UniProt (by SIFTS) (P42212)	Aequorea victoria	Transmembrane protein 76
2	C, G (A, B)	ACETYL COENZYME *A	non-polymer		809.6	2	Chemie (ACO)
3	D, E, F, H, I... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B: -255 - -13 (UniProt: A0A9X4KGN5)

PDB	External Database	Details
Met -267	-	initiating methionine
Trp -266	-	expression tag
Ser -265	-	expression tag
His -264	-	expression tag
Pro -263	-	expression tag
Gln -262	-	expression tag
Phe -261	-	expression tag
Glu -260	-	expression tag
Lys -259	-	expression tag
Gly -258	-	expression tag
Ser -257	-	expression tag
Gly -256	-	expression tag
Lys -50	Ala 214	conflict
Gly -12	-	linker
Leu -11	-	linker
Glu -10	-	linker
Val -9	-	linker
Leu -8	-	linker
Phe -7	-	linker
Gln -6	-	linker
Gly -5	-	linker
Pro -4	-	linker
Glu -3	-	linker
Phe -2	-	linker
Glu -1	-	linker
Phe 0	-	linker

A, B: 1 - 635 (UniProt: Q68CP4)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	201634.3
Non-Polymers*	Number of molecules	8
	Total formula weight	2946.4
All*	Total formula weight	204580.7

*Water molecules are not included.