8TU9
Cryo-EM structure of HGSNAT-acetyl-CoA complex at pH 7.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003674 | molecular_function | molecular_function |
| A | 0005575 | cellular_component | cellular_component |
| A | 0005764 | cellular_component | lysosome |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006091 | biological_process | generation of precursor metabolites and energy |
| A | 0007041 | biological_process | lysosomal transport |
| A | 0008218 | biological_process | bioluminescence |
| A | 0015019 | molecular_function | heparan-alpha-glucosaminide N-acetyltransferase activity |
| A | 0016020 | cellular_component | membrane |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0030200 | biological_process | heparan sulfate proteoglycan catabolic process |
| A | 0035579 | cellular_component | specific granule membrane |
| A | 0043202 | cellular_component | lysosomal lumen |
| A | 0051259 | biological_process | protein complex oligomerization |
| A | 0070821 | cellular_component | tertiary granule membrane |
| B | 0003674 | molecular_function | molecular_function |
| B | 0005575 | cellular_component | cellular_component |
| B | 0005764 | cellular_component | lysosome |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006091 | biological_process | generation of precursor metabolites and energy |
| B | 0007041 | biological_process | lysosomal transport |
| B | 0008218 | biological_process | bioluminescence |
| B | 0015019 | molecular_function | heparan-alpha-glucosaminide N-acetyltransferase activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0030200 | biological_process | heparan sulfate proteoglycan catabolic process |
| B | 0035579 | cellular_component | specific granule membrane |
| B | 0043202 | cellular_component | lysosomal lumen |
| B | 0051259 | biological_process | protein complex oligomerization |
| B | 0070821 | cellular_component | tertiary granule membrane |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 584 |
| Details | TOPO_DOM: Lumenal, vesicle => ECO:0000255 |
| Chain | Residue | Details |
| A | MET1-ASP190 | |
| B | ILE442-THR500 | |
| B | PHE551-LYS564 | |
| B | VAL614-LYS634 | |
| A | MET297-GLN302 | |
| A | ALA367-CYS374 | |
| A | ILE442-THR500 | |
| A | PHE551-LYS564 | |
| A | VAL614-LYS634 | |
| B | MET1-ASP190 | |
| B | MET297-GLN302 | |
| B | ALA367-CYS374 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 400 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| A | ASP191-SER211 | |
| A | PHE593-ILE613 | |
| B | ASP191-SER211 | |
| B | THR276-SER296 | |
| B | ARG303-CYS323 | |
| B | PRO346-PHE366 | |
| B | ALA375-LEU395 | |
| B | GLY421-TYR441 | |
| B | LYS501-LEU521 | |
| B | PHE530-ALA550 | |
| B | GLY565-GLY585 | |
| A | THR276-SER296 | |
| B | PHE593-ILE613 | |
| A | ARG303-CYS323 | |
| A | PRO346-PHE366 | |
| A | ALA375-LEU395 | |
| A | GLY421-TYR441 | |
| A | LYS501-LEU521 | |
| A | PHE530-ALA550 | |
| A | GLY565-GLY585 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 242 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000255 |
| Chain | Residue | Details |
| A | GLU212-LEU275 | |
| B | HIS586-TYR592 | |
| A | ILE324-ILE345 | |
| A | ILE396-LEU420 | |
| A | THR522-GLY529 | |
| A | HIS586-TYR592 | |
| B | GLU212-LEU275 | |
| B | ILE324-ILE345 | |
| B | ILE396-LEU420 | |
| B | THR522-GLY529 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:20650889 |
| Chain | Residue | Details |
| A | MET297 | |
| B | MET297 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | VAL243 | |
| B | VAL243 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR245 | |
| B | THR245 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | GLY94 | |
| A | SER162 | |
| B | GLY94 | |
| B | SER162 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218 |
| Chain | Residue | Details |
| A | ILE142 | |
| B | ILE142 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: (Z)-2,3-didehydrotyrosine => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| A | TYR-190 | |
| B | TYR-190 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | CROSSLNK: 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| A | THR-191 | |
| A | GLY-189 | |
| B | THR-191 | |
| B | GLY-189 |
