8TTB

Cryo-EM structure of the PP2A:B55-ARPP19 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform	polymer	584	64958.0	1	UniProt (P30153) Pfam (PF02985)	Homo sapiens (human)	Medium tumor antigen-associated 61 kDa protein,PP2A subunit A isoform PR65-alpha,PP2A subunit A isoform R1-alpha
2	B (B)	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform	polymer	450	52044.3	1	UniProt (P63151)	Homo sapiens (human)
3	C (C)	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform	polymer	311	35845.4	1	UniProt (P67775) Pfam (PF00149)	Homo sapiens (human)	PP2A-alpha,Replication protein C,RP-C
4	D (D)	cAMP-regulated phosphoprotein 19	polymer	114	12620.2	1	UniProt (P56211) Pfam (PF04667)	Homo sapiens (human)
5	E (C)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
6	F (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 9 - 589 (UniProt: P30153)

PDB	External Database	Details
Gly 6	-	expression tag
His 7	-	expression tag
Met 8	-	expression tag

B: 2 - 447 (UniProt: P63151)

PDB	External Database	Details
His -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

C: 1 - 309 (UniProt: P67775)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag

D: 1 - 112 (UniProt: P56211)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Ala 104	Ser 104	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165467.9
Non-Polymers*	Number of molecules	2
	Total formula weight	121.3
All*	Total formula weight	165589.1

*Water molecules are not included.