8TR3

Cryo-EM structure of HmAb64 scFv in complex with CNE40 SOSIP trimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, C, E)	CNE40 SOSIP Envelope glycoprotein gp120	polymer	489	55018.4	3	UniProt (D7S2E5) Pfam (PF00516)	Human immunodeficiency virus 1
2	B, F, J (B, D, F)	CNE40 SOSIP Transmembrane protein gp41	polymer	153	17205.5	3	UniProt (D7S2E5) Pfam (PF00517)	Human immunodeficiency virus 1
3	C, G, K (H, I, J)	HmAb64 Fv heavy chain	polymer	124	13745.4	3		Homo sapiens
4	D, H, L (L, M, N)	HmAb64 Fv light chain	polymer	109	11852.1	3		Homo sapiens
5	M, N, O, P, Q... (P, R, c, e, p...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
6	AA, BA, CA, DA, EA... (A, C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)

Sequence modifications

A, C, E: 32 - 511 (UniProt: D7S2E5)

PDB	External Database	Details
Cys 501	Ala 507	engineered mutation
Arg 502	Lys 508	engineered mutation
Arg 509	Glu 515	engineered mutation
Arg 510	Lys 516	engineered mutation
Arg 512	-	insertion
Arg 513	-	insertion

B, D, F: 512 - 664 (UniProt: D7S2E5)

PDB	External Database	Details
Pro 559	Ile 565	engineered mutation
Cys 605	Thr 611	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	293464.2
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	27
	Total formula weight	5972.6
All*	Total formula weight	301983.2

*Water molecules are not included.