8TQZ

Eukaryotic translation initiation factor 2B with a mutation (L516A) in the delta subunit

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (G, H)	Translation initiation factor eIF-2B subunit alpha	polymer	322	35723.2	2	UniProt (Q14232) Pfam (PF01008)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit alpha
2	C, G (A, B)	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80452.6	2	UniProt (Q13144) Pfam (PF02020)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit epsilon
3	D, H (D, C)	Translation initiation factor eIF-2B subunit beta	polymer	368	41008.6	2	UniProt (P49770) Pfam (PF01008)	Homo sapiens (human)	S20I15,S20III15,eIF-2B GDP-GTP exchange factor subunit beta
4	E, I (E, F)	Translation initiation factor eIF-2B subunit delta	polymer	523	57598.1	2	UniProt (Q9UI10) Pfam (PF01008)	Homo sapiens (human)
5	F, J (I, J)	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50) Pfam (PF00483) Pfam (PF25084)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit gamma

Sequence modifications

G, H: 2 - 305 (UniProt: Q14232)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag

A, B: 1 - 721 (UniProt: Q13144)

PDB	External Database	Details
Val 587	Ile 587	conflict

D, C: 2 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag

E, F: 1 - 523 (UniProt: Q9UI10)

PDB	External Database	Details
Ala 516	Leu 516	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	530173.3
All*	Total formula weight	530173.3

*Water molecules are not included.