8TL6

Cryo-EM structure of DDB1deltaB-DDA1-DCAF5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA damage-binding protein 1	polymer	864	96425.6	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B (B)	DDB1- and CUL4-associated factor 5	polymer	986	108948.7	1	UniProt (Q96JK2) Pfam (PF00400)	Homo sapiens (human)	Breakpoint cluster region protein 2,BCRP2,WD repeat-containing protein 22
3	C (E)	DET1- and DDB1-associated protein 1	polymer	126	14542.2	1	UniProt (Q9BW61) Pfam (PF10172)	Homo sapiens (human)	Placenta cross-immune reaction antigen 1,PCIA-1

Sequence modifications

A: 1 - 699 (UniProt: Q16531)

PDB	External Database	Details
Met -27	-	initiating methionine
Gly -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
His -14	-	expression tag
Ile -13	-	expression tag
Val -12	-	expression tag
Met -11	-	expression tag
Val -10	-	expression tag
Asp -9	-	expression tag
Ala -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Pro -5	-	expression tag
Thr -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

A: 706 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 700	-	linker
Asn 701	-	linker
Gly 702	-	linker
Asn 703	-	linker
Ser 704	-	linker
Gly 705	-	linker

B: 1 - 942 (UniProt: Q96JK2)

PDB	External Database	Details
Met -43	-	initiating methionine
Asp -42	-	expression tag
Trp -41	-	expression tag
Ser -40	-	expression tag
His -39	-	expression tag
Pro -38	-	expression tag
Gln -37	-	expression tag
Phe -36	-	expression tag
Glu -35	-	expression tag
Lys -34	-	expression tag
Ser -33	-	expression tag
Ala -32	-	expression tag
Val -31	-	expression tag
Gly -30	-	expression tag
Leu -29	-	expression tag
Asn -28	-	expression tag
Asp -27	-	expression tag
Ile -26	-	expression tag
Phe -25	-	expression tag
Glu -24	-	expression tag
Ala -23	-	expression tag
Gln -22	-	expression tag
Lys -21	-	expression tag
Ile -20	-	expression tag
Glu -19	-	expression tag
Trp -18	-	expression tag
His -17	-	expression tag
Glu -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

E: 1 - 102 (UniProt: Q9BW61)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	219916.4
All*	Total formula weight	219916.4

*Water molecules are not included.