8TIA

CryoEM structure of locally-refined tetramer of Shedu nuclease domain from Bacillus cereus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Shedu protein SduA	polymer	229	26236.8	4	UniProt (B7HFR2)	Bacillus cereus B4264	Putative nuclease SduA

Sequence modifications

A, B, C, D: 171 - 380 (UniProt: B7HFR2)

PDB	External Database	Details
Met 152	-	expression tag
Lys 153	-	expression tag
Ser 154	-	expression tag
Ser 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
Glu 162	-	expression tag
Asn 163	-	expression tag
Leu 164	-	expression tag
Tyr 165	-	expression tag
Phe 166	-	expression tag
Gln 167	-	expression tag
Ser 168	-	expression tag
Asn 169	-	expression tag
Ala 170	-	expression tag
Ala 264	Glu 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	104947.0
All*	Total formula weight	104947.0

*Water molecules are not included.