8TI9

CryoEM structure of octamer assembly of Shedu nuclease domain from Bacillus cereus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E, F, G, H, A...	Shedu protein SduA	polymer	229	26236.8	8	UniProt (B7HFR2) Pfam (PF14082) In PDB	Bacillus cereus B4264	Putative nuclease SduA

Sequence modifications

E, F, G, H, A, B, C, D: 171 - 380 (UniProt: B7HFR2)

PDB	External Database	Details
Met 152	-	expression tag
Lys 153	-	expression tag
Ser 154	-	expression tag
Ser 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
Glu 162	-	expression tag
Asn 163	-	expression tag
Leu 164	-	expression tag
Tyr 165	-	expression tag
Phe 166	-	expression tag
Gln 167	-	expression tag
Ser 168	-	expression tag
Asn 169	-	expression tag
Ala 170	-	expression tag
Ala 264	Glu 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	209894.1
All*	Total formula weight	209894.1

*Water molecules are not included.