8TH4

Structure of AT118-L Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor and Losartan

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Type-1 angiotensin II receptor Soluble cytochrome b562 complex	polymer	433	49501.1	1	UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1 receptor,Cytochrome b-562
2	B (C)	BAG2 Anti-BRIL Fab Heavy Chain	polymer	231	24539.3	1		synthetic construct
3	C (D)	BAG2 Anti-BRIL Fab Light Chain	polymer	215	23541.2	1		synthetic construct
4	D (B)	AT118-L Nanobody	polymer	140	15345.1	1		synthetic construct
5	E (A)	[2-butyl-5-chloranyl-3-[[4-[2-(2H-1,2,3,4-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol	non-polymer		422.9	1	Chemie (LSN)

Sequence modifications

A: 2 - 226 (UniProt: P30556)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

A: 227 - 330 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 232	Met 29	engineered mutation
Ile 327	His 124	engineered mutation
Leu 331	-	linker
Glu 332	-	linker
Arg 333	-	linker
Ala 334	-	linker
Arg 335	-	linker
Ser 336	-	linker
Thr 337	-	linker
Leu 338	-	linker
Asp 339	-	linker
Lys 340	-	linker
Leu 341	-	linker

A: 1235 - 1319 (UniProt: P30556)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112926.6
Non-Polymers*	Number of molecules	1
	Total formula weight	422.9
All*	Total formula weight	113349.5

*Water molecules are not included.